Methods of exfoliation using N-acetyl glucosamine

Drug – bio-affecting and body treating compositions – Preparations characterized by special physical form – Cosmetic – antiperspirant – dentifrice

Reexamination Certificate

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C424S078030, C514S062000, C514S886000, C514S937000, C514S944000

Reexamination Certificate

active

06413525

ABSTRACT:

FIELD OF THE INVENTION
The present invention relates to methods of substantially exfoliating the skin. In particular, the invention relates to topically applied compositions containing an amino sugar in the form of N-acetyl glucosamine.
BACKGROUND OF THE INVENTION
This invention relates to methods of using N-acetyl glucosamine to exfoliate the skin. The cells of the outermost layer of the stratum corneum are constantly shed naturally, by the normal process of desquamation, as minute particles. When fully keratinised tissue loses its cellular structure and reaches the surface of the stratum corneum, it breaks up into microscopic squames and sheds off the surface of the skin. Microscopic squames at the skin surface are commonly referred to as dead skin cells and make up a dead skin layer on the skin surface. The process of desquamation has been estimated to cause a loss of tissue in an amount of up to 14 grams per day. This loss is constantly replenished with cells from lower layers of the epidermis. Thus, the layers of the epidermis are composed of cells moving up towards the surface in successive stages of differentiation until death when they are finally sloughed off and lost to the environment. Desquamation is one of the processes by which skin maintains its health and vitality as nutrients and moisture are continuously replaced on the surface of the skin when dead skin cells are removed. Normally, the desquamation process takes about 14 days (i.e., the corneocyte takes 14 days to reach the outermost layer of the strateum corneum to be shed). When desquamation does not take place regularly, the surface of the skin tends to become rougher and more wrinkles and other undesirable effects appear on the surface of the skin. However, in addition to or as an alternative to the natural desquamation process, exfoliation is often used to rejuvenate and enhance the health of the skin.
Exfoliation is a technique whereby dead skin cells are removed or sloughed from the skin surface to promote a healthier and more youthful appearance to the skin. Several compounds are known to be useful as exfoliants such as for example, alpha hydroxy acid (“AHA”), beta hydroxy acid (“BHA”), retinoic acid (“retin A”), and enzymes. An exfoliant such as AHA breaks the bond holding individual squames together and allows them to detach and shed.
Producing a variety of alternatives for exfoliation is desirable because skin types vary among consumers and therefore, having a variety of exfoliants available to meet various individual skincare needs of consumers is beneficial. Thus, there is a continued effort to find additional alternative ways of aiding the sloughing ability of the skin and promoting its health for various types of skin. This is, therefore, an object of the present invention.
SUMMARY OF THE INVENTION
The present invention relates to a method for exfoliating the skin by topically applying pharmaceutical or cosmetic compositions containing an exfoliating-effective amount of N-acetyl-D-glucosamine, N-acetylgalactosamine, or a combination thereof. This method provides a natural alternative to the exfoliation process that is as effective as other typical exfoliation techniques.
DETAILED DESCRIPTION OF THE INVENTION
It has now been unexpectedly discovered that topically applied N-acetyl-D-glucosamine or N-acetylgalactosamine containing compositions exfoliate the skin. Combinations of N-acetyl-D-glucosamine and N-acetylgalactosamine can also be used. These compositions which exfoliate the skin can also include chitin to enhance the process of exfoliation.
It is known that amino sugars are capable of modulating the adhesion of keratinocytes, in vitro. It is disclosed by Brysk, M. in “Glycoproteins modulate adhesion in terminally differentiated keratinocytes,” that N-acetylglucosamine, N-acetylneuraminic acid and N-acetylgalactosamine are involved in the dissociation of aggregates of glycoproteins that bind corneocytes together. Specifically, amino sugars are known to inhibit the reaggregation of corneocytes which have been dissociated into single squames by homogenization in ether. Further, amino sugars in the form of N-acetylglucosamine have been used as an oral nutrient supplement in conjunction with other topical cosmetic products, such as for example, products offered by Life-Force, Inc. which include N-acetyl-D-glucosamine in the form of a pill taken as a nutritional supplement. However, it is not suggested in the prior art to formulate a pharmaceutical or cosmetic composition for topical application to the skin using N-acetylglucosamine as an exfoliant. In fact, in WO97/12597 it is disclosed that when studied to determine its efficacy as a topical desquamating agent, N-acetyl-D-glucosamine was not found to be effective. The exfoliant in WO97/12597 is a compound comprising a chain of carbohydrates linked by a linking moiety to an alkyl or alkenyl chain. Further, WO97/12597, incorporated herein by reference, only includes N-acetyl-D-glucosamine as one of the units forming the carbohydrate portion which is linked by the linking moiety to the alkyl or alkenyl chain for desquamation of the skin. It is not disclosed in WO97/12597 that N-acetyl-D-glucosamine alone can exfoliate the skin.
Compositions for topical application containing N-acetyl-D-glucosamine have been disclosed for example, in JP 59013708, WO 98/152576, and U.S. Pat. No. 5,866,142, each incorporated herein by reference. To soften and moisturize the skin, a cosmetic containing an N-acetyl amino sugar is disclosed in JP 59013708. A composition for alleviating itching and pain containing N-acetyl-D-glucosamine is disclosed in WO 98/52576. In U.S. Pat. No. 5,866,142, a composition for exfoliating the skin has been disclosed, which includes N-acetyl-D-glucosamine. The presence of N-acetyl-D-glucosamine contributes to enhancing the amount of hyaluronic acid which the skin naturally produces in greater quantities in response to exfoliation, induced by other compounds such as histidine. However, no exfoliating activity is attributed to N-acetyl-D-glucosamine. A cosmetic composition containing N-acetylglucosamine and having good adhesion to the skin (i.e., does not lift off of the skin) is disclosed in JP 8188526. However, the prior art does not disclose the ability of glucosamine, and particularly, N-acetyl-D-glucosamine, to exfoliate the skin.
The composition of the present invention contains an exfoliating-effective amount of N-acetyl-D-glucosamine, N-acetylgalactosamine, or a combination thereof. By the term “exfoliating-effective amount,” as used in the present specification, is meant an amount which is effective to cause exfoliation of the skin. The amount of N-acetyl-D-glucosamine, N-acetylgalactosamine, or a combination thereof, in the present invention will vary depending on the desired strength or intensity of exfoliation. The N-acetyl-D-glucosamine or N-acetylgalactosamine is present in the composition in an amount of about 0.01 to about 25.0 percent of the weight of the composition; preferably 0.5 to about 10.0 percent, and more preferably about 1.0 to 5.0 percent of the weight of the composition.
The N-acetyl-D-glucosamine or N-acetylgalactosamine per se is added directly to the cosmetic or pharmaceutical composition by admixing; alternatively, extracts of materials containing substantial quantities of N-acetylglucosamine or N-acetylgalactosamine as a component can be used to provide the same concentration. These compositions can also contain chitin. The amounts of chitin in the composition are also present in exfoliating-effective amounts as previously described.
The intensity of exfoliation can also be controlled by the frequency with which the compositions are applied to the skin and the compositions are applied periodically for a period of time sufficient to exfoliate the skin. Accordingly, the compositions are applied to the skin for a period of at least 2 months, and preferably for at least 4 months, during which time the compositions are applied on a weekly basis. However, a preferred method of obtaining the benefits of the co

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