Methods of ester hydrolysis

Details Methods of ester hydrolysis Methods of ester hydrolysis

1992-06-22

1993-09-14

Low, Christopher S. F.

Chemistry: molecular biology and microbiology

Micro-organism, tissue cell culture or enzyme using process...

Enzymatic production of a protein or polypeptide

435219, 435222, 435220, 435221, 930240, 935 14, C12P 2102

Patent

active

052447910

ABSTRACT:
A novel method for ester hydrolysis catalyzed by enzymes having an oxyanion hole wherein the amide hydrolysis reaction is minimized. Enzymes are selected or alternatively derived by replacement of amino acid residues, which have minimal hydrogen bonding at or within 15 angstroms of the oxyanion hole.

REFERENCES:
patent: 4086136 (1928-04-01), Isowa et al.
patent: 4760025 (1988-07-01), Estell et al.
patent: 4800162 (1989-01-01), Matoor
patent: 5002871 (1991-07-01), Iacobucci
Polgar et al. 1970. Adv. Enzymol. 33, 381-400.
Wells et al. 1983. Nuc. Acids. Res. 11, 7911-7923.
Wallace et al. 1981. Nuc. Acids. Res. 9, 3647-3656.
Rastetter, W. 1983. Trends Biotechnol. 1, 80-84.
Svendsen, I. 1976. Carlsberg. Res. Commun. 41, 237-291.
Robertus et al. 1972. Biochemistry. 11, 2439-2449.
Wells et al. 1986. Phil. Trans. R. Soc. Lond. 317, 415-423.
Breddam et al., 1980. Carlsberg Res. Commun. 45, 237-247.
Reich et al. (eds.) 1975. in: Proteases and Biological Control. Cold Spring Harbor Laboratory. Cold Spring Harbor; N.Y. pp. 1-11.
Phillip et al. 1983. Molec. Cell. Biochem. 51, 5-32.
Matthews et al. 1975. J. Biol. Chem. 250, 7120-7126.

Affiliated with

Also associated with

Rating

Methods of ester hydrolysis does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Methods of ester hydrolysis, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Methods of ester hydrolysis will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFUS-PAI-O-2025905