Chemistry: molecular biology and microbiology – Process of utilizing an enzyme or micro-organism to destroy... – Resolution of optical isomers or purification of organic...
Patent
1992-09-03
1994-08-30
Robinson, Douglas W.
Chemistry: molecular biology and microbiology
Process of utilizing an enzyme or micro-organism to destroy...
Resolution of optical isomers or purification of organic...
435135, 435198, C07C 6700, C12P 762, C12N 920
Patent
active
053427807
ABSTRACT:
Methods of separating stereoisomers of a racemic mixtures of a compound employ porcine pancreatic lipase which selectively transesterfies one enantiomer of an ester function which is proximate to a chiral isomeric site are presented. The compound is a reactive ester of the formula: ##STR1## The method comprises obtaining a racemic mixture of the reactive ester, reacting the reactive ester with a poly(ethylene glycol) of average molecular weight of about 100 to about 10,000 Daltons and the enzyme. The reactive ester, the poly(ethylene glycol) and the enzyme are reacted in a medium in proportions other conditions effective to form a chiral site proximate poly(ethylene glycol) ester. The chiral site approximate poly(ethylene glycol) ester is then separated from the reaction medium, the reactive ester, the poly(ethylene glycol) and the enzyme.
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Morrow Cary J.
Wallace Joe S.
Robinson Douglas W.
University of New Mexico
Weber Jon P.
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