Methods of enhanced heterologous protein secretion

Details Methods of enhanced heterologous protein secretion Methods of enhanced heterologous protein secretion

2008-09-12

2011-10-11

Desai, Anand (Department: 1656)

Chemistry: molecular biology and microbiology

Micro-organism, per se ; compositions thereof; proces of...

Fungi

C435S069600, C435S224000, C435S483000, C536S063000, C536S063000, C536S063000

Reexamination Certificate

active

08034607

ABSTRACT:
A method of enhancing heterologous protein secretion in a yeast cell is disclosed. In one embodiment, the method comprising the steps of engineering a yeast cell to overexpress at last one gene selected from the group consisting of CCW12, CWP2, SED1, RPP0, ERO1 and their homologs, supplying the yeast cell with a nucleic acid encoding a heterologous protein and obtaining increased expression of the heterologous protein, wherein the expression is increased relative to the protein expression in a yeast cell that does not overexpress a gene selected from the group consisting of CCW12, CWP2, SED1, RPP0, ERO1 and their homologs.

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