Chemistry: molecular biology and microbiology – Apparatus – Including measuring or testing
Reexamination Certificate
2005-10-11
2005-10-11
Falk, Anne-Marie (Department: 1636)
Chemistry: molecular biology and microbiology
Apparatus
Including measuring or testing
C536S023100
Reexamination Certificate
active
06953686
ABSTRACT:
The present invention provides methods for the purification of plasmid DNA that includes removal of host cell impurities, such as endotoxins, RNA, proteins, and chromosomal DNA, from an aqueous solution containing plasmid DNA and methods for separation and purification of supercoiled plasmid DNA from an aqueous solution containing a mixture of supercoiled and nicked or relaxed plasmid DNA using hydrophobic interaction chromatography supports.
REFERENCES:
patent: 6197553 (2001-03-01), Lee et al.
patent: 6214586 (2001-04-01), McNeilly
patent: 6265168 (2001-07-01), Gjerde et al.
patent: 6441160 (2002-08-01), Kitamura et al.
patent: 2001/0034435 (2001-10-01), Nochumson et al.
patent: 0 964 057 (1999-12-01), None
Prazeres et al. Preparative purification of supercoiled plamsid DNA using anion-exchange chromatography, J. Chromatography. vol. 806:31-45, 1998.
Ishida et al. Chromatographic removal of endotoxin from a macromolecular intitumor antibiotic SN-07. Kagaku Kogaku Rondunshu vol. 17(3):589-594, 1991.
Maitra et al. Properties of binding ofEscherichia coliendotoxin to various matrices. J. Clin Microbiol. vol. 13(1):49-53, Jan. 1981.
Colote et al. Analysis and purificatoin of plasmid DNA by reversed-phase high-performance liquid chromatography. Analytical Biochem. vol. 154:15-20, 1986.
Kapp et al. Preparation of DNA topoisomers by RP-18 high-performance liquid chromatography. Anal. Biochem. vol. 206:293-299, 1992.
Saha et al, “A new method of plasmid DNA . . . ”, Anal Biochem Feb. 1, 1989;176(2):344-9.
Ranhand, “The enrichment of plasmid DNAs, . . . ”, Prep Biochem 1985;15(3):121-31.
Sayers et al, “Identification and eradication . . . ”, Anal Biochem Oct. 15, 1996;241(2):186-9.
Cooke et al, “Purification of essentially RNA free . . . ”, J Biotechnol Feb. 23, 2001;85(3):297-304.
O'Kennedy et al, “Effects of growth medium selection . . . ”, J Biotechnol Jan. 21, 2000;76(2-3):175-83.
Smith et al, “Fast and accurate method . . . ”, Biotechniques Mar. 1999;26(3):518-22, 524, 526.
Chicz et al, “Microenvironmental contributions . . . ”, J Chromatogr Feb. 2, 1990;500:503-18.
Smith et al, “Effect of stationary and mobile phases . . . ”, J Chromatogr Nov. 10, 1989;496(1):71-82.
Fausnaugh et al, “Comparison of hydrophobic-interaction . . . ”, J Chromatogr Dec. 28, 1984;317-:141-55.
Alpert, “Hydrophobic interaction chromatography . . . ”, J Chromatogr Jul. 1, 1988;444:269-74.
Amersham Pharmacia Biosciences Catalog 2001 pp. 644 and 650.
“Reversed-Phase and Hydrophobic Interaction Chromatography of Peptides and Proteins”, El Rassi et al, Yale University, New Haven, CT, pp. 447-494, in Separation Processes in Biotechnology, edited by Juan A. Asenjo, 1990. -Full Article Submitted.
Edwardson et al, “A new rapid procedure for the prparation of plasmid DNA”, Anal Biochem Feb. 1, 1986;152(2):215-20.
Diogo et al, “Purification of a cystic fibrosis plasmid vector for gene therapy using hydrophobic interaction chromatography”, Biotchnol Bioeng Jun. 5, 2000;68(5):576-83, abstract only.
Chaturvedi et al, “The delta-endotoxin proteins accumulate inEscherichia colias a . . . ”, Protein Expr Purif Oct. 2000; 20(1):21-6, abstract only.
Sparks et al, “A simple and rapid procedure for the purification of plasmid DNA . . . ”, Anal Biochem Dec. 1983; 135(2):345-8, abstract only.
Santosa, “Rapid extraction and purification of environmental DNA . . . ”, Mol Biotechnol Jan. 2001; 17(1):59-64, abstract only.
Ihle et al, “Efficient purification of DNA fragments using . . . ”, Nucleic Acids Res Aug. 15, 2000; 28(16):E76, abstract only.
Kahn et al, “Purification of plasmid DNA by tangential . . . ”, Biotechnol Bioeng Jul. 5, 2000;69(1):101-6, abstract only.
Ferreira et al, “Anion exchange purification of plasmid DNA . . . ”, Bioseparation 2000;9(1);1-6, abstract only.
Madiraju et al, “Development of simple and efficient protocol . . . ”, Lett Appl Microbiol Jan. 2000;30(1):38-41, abstract only.
Levy et al, “Removal of contaminant nucleic acids by nitrocellulose . . . ”, J. Biotechnol Jan. 21, 2000; 76(2-3):197-205, abstract only.
Limor et al, “Contaminant eluted from solid-phase plasmid . . . ”, J Biochem Biophys Methods Jul. 28, 1999; 40(1-2):57-64, abstract only.
Ferrus et al, “A rapid procedure for the isolation . . . ”, Int Microbiol Jun. 1999;2(2):115-7, abstract only.
Itoh et al, “Automated filtration-based high-throughput . . . ”, Genome Res May 1999; 9(5):463-70, abstract only.
Schluep et al, “Purification of plasmids by triplex . . . ”, Nucleic Acids Res Oct. 1, 1998;26(19):4524-8, abstract only.
Levison et al, “Recent developments of magnetic beads . . . ”, J Chromatogr A Aug. 7, 1998;816(1):107-11, abstract only.
Prazeres et al, “Preparative purification of supercoiled . . . ”, J Chromatogr A May 8, 1998;806(1):31-45, abstract only.
LaMarr et al, “Large Scale preparation of positively supercoiled . . . ”, Nucleic Acids Res Apr. 15, 1997;25(8):1660-1, abstract only.
Itoh et al, “Simple and rapid preparation of plasmid . . . ”, Nucleic Acids Res Mar. 15, 1997;25(6):1315-6, abstract only.
Davis et al, “Comparison of plasmid DNA preparation . . . ”, Biotechniques Jul. 1996;21(1):92-4, 96-9, abstract only.
Pham et al, “Preparation of pure plasmid or cosmid . . . ”, Biotechniques Mar. 1996;20(3):492-7, abstract only.
Ruppert et al, “A filtration method for plasmid . . . ”, Anal Biochem Sep 1, 1995;230(1):130-4, abstract only.
Moser et al, “Plasmid and chromosomal DNA . . . ”, FEMS Microbiol Lett May 15, 1995;128(3):307-13. abstract only.
Horn et al, “Cancer gene therapy using . . . ”, Hum Gene Ther May 1995;6(5):565-73, abstract only.
Wicks et al, “Bacterial lipopolysaccharide . . . ”, Hum Gen Ther Mar. 1995;6(3):317-23, abstract only.
Tarczynski et al, “Two-minute miniprep . . . ”, Biotechniques Mar. 1994;16(3):514-9, abstract only.
Wang et al, “Large-scale supercoiled plasmid preparation . . . ”, Biotechniques Mar. 1994;16(3):460-3.
Feliciello et al, “A modified alkaline lysis method for the . . . ”, Anal Biochem Aug. 1, 1993;212(2):394-401.
Chakrabarti et al, “A procedure for large-scale plasmid . . . ”, Biotechnol Appl Biochem Oct. 1992;16(2):211-5.
Azad et al, “An improvied method for rapid purification . . . ”, Lett Appl Microbiol Jun. 1992;14(6):250-4.
Chandra et al, “Large-scale purification of plasmid DNA . . . ”, Anal Biochem May 15, 1992;203(1):169-72.
Cole, “Purification of plasmid and high molecular . . . ”, Biotechniques Jul. 1991;11(1):18, 20, 22-4.
He et al, “An improved and rapid procedure . . . ”, Genet Anal Tech Appl May 1991;8(3):107-10.
Ishaq et al, “Large-scale isolation of plasmid . . . ”, Biotechniques Jul. 1990;9(1):19-20, 22, 24.
McClung et al, “Purification of plasmid DNA by fast protein . . . ”, Anal Biochem Mar. 1989;177(2):378-82.
LeBrun et al, “A simple method for the preparation of . . . ”, Biotechniques Oct. 1988;6(9):834, 837-8.
Moreau et al, “Purification and separation of various . . . ”, Anal Biochem Oct. 1987;166(1):188-93.
Lev, “A procedure for large-scale isolation of RNA . . . ”, Analy Biochem Feb. 1, 1987:160(2):332-6.
Gomez-Marquez et al, “A simple procedure for large-scale . . . ”, Gene 1987;54(2-3):255-9.
Monstein et al, “A rapid and inexpensive method for . . . ”, Biochem Int Jun. 1986;12(6):889-96.
Naumov, “Purification of plasmid DNA by chromatographic methods”, Mol Gen Mikrobiol Virusol 1985 Sep.;(9):44-7.
Mukhopadhyay et al, “A simple procedure for large-sca
Cambrex Bio Science Baltimore, Inc.
Falk Anne-Marie
Nixon & Vanderhye P.C.
Qian Celine
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