Chemistry: molecular biology and microbiology – Enzyme – proenzyme; compositions thereof; process for... – Oxidoreductase
Reexamination Certificate
2003-08-22
2008-08-26
Kam, Chih-Min (Department: 1656)
Chemistry: molecular biology and microbiology
Enzyme , proenzyme; compositions thereof; process for...
Oxidoreductase
C435S069100, C435S320100, C435S325000, C536S023200
Reexamination Certificate
active
07416870
ABSTRACT:
The present invention provides methods of modifying a biological molecule by C—O bond formation utilizing a type II polyketide synthase (PKS) system from the nonactin biosynthesis gene cluster. The type II PKS responsible for biosynthesis of the macrotetralide nonactin includes polypeptides encoded by the nonJK genes. The NonJ and NonK polypeptides have been identified by the inventors as ketoacyl synthases capable of directly catalyzing C—O bond formation between substrate molecules. This invention increases the scope and diversity of chemical syntheses available for drug design and combinatorial biosynthesis.
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Kwon Hyung-Jin
Shen Ben
Fulbright & Jaworski LLP
Kam Chih-Min
Wisconsin Alumni Research Foundation
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