Chemistry: molecular biology and microbiology – Animal cell – per se ; composition thereof; process of... – Method of regulating cell metabolism or physiology
Reexamination Certificate
2011-04-05
2011-04-05
Falk, Anne-Marie (Department: 1632)
Chemistry: molecular biology and microbiology
Animal cell, per se ; composition thereof; process of...
Method of regulating cell metabolism or physiology
C435S325000, C435S366000, C435S368000, C435S455000
Reexamination Certificate
active
07919317
ABSTRACT:
The present invention provides a method of differentiating progenitor cells to produce a population containing protected neuronal cells. A method of the invention includes the steps of contacting the progenitor cells with a differentiating agent; and introducing into the progenitor cells a nucleic acid molecule encoding a MEF2 polypeptide or an active fragment thereof, thereby differentiating the progenitor cells to produce a population containing protected neuronal cells. In one embodiment, the MEF2 polypeptide is human MEF2C or an active fragment thereof.
REFERENCES:
patent: 5843780 (1998-12-01), Thomson
patent: 6153591 (2000-11-01), Cai et al.
patent: 6200969 (2001-03-01), Fritz et al.
Eiges et al. (2001) Establishment of human embryonic stem cell-transfected clones carrying a marker for undifferentiated cells. Current Biology 11:514-518.
Hanazono et al. (2001) Gene transfer into nonhuman primate hematopoietic stem cells: Implications for gene therapy.
Jackowski, A. (1995) Neural injury repair: hope for the future as barriers to effective CNS regeneration become clearer. British J. Neurosurgery 9: 303-317.
Zwaka et al. (2003) Homologous recombination in human embryonic stem cells. Nature Biotechnology, advanced online publication, Feb. 10, 2003, pp. 1-3.
Cao et al. (2002) Stem cell repair of central nervous system injury. J. Neuroscience Research 68: 501-510.
Mehler et al. (1999) Progenitor cell biology: Implications for neural regeneration. Archives of Neurology 56: 780-784.
Milward et al. (1997) Isolation and transplantation of multipotential populations of epidermal growth factor-responsive, neural progenitor cells from the canine brain. J. Neuroscience Research 50: 862-871.
Rossi and Cattaneo (2002) Neural stem cell therapy for neurological diseases: dreams and reality. Nature Reviews Neuroscience 3: 401-409.
Filbin, MT (2003) Myelin-associated inhibitors of axonal regeneration in the adult mammalian CNS. Nature Reviews 4: 1-11.
Grados-Munro et al. (2003) Myelin-associated inhibitors of axon regeneration. J. Neurosci. Res. 74: 479-485.
Krainc et al. (1998) Synergistic activation of the N-methyl-D-aspartate receptor subunit 1 promoter by myocyte enhancer factor 2C and Sp1. J. Biol. Chem. 273(40): 26218-26224.
Skerjanc et al. (1998) Myocyte enhancer factor 2C and Nkx2-5 up-regulate each other's expression and initiate cardiomyogenesis in P19 cells. J. Biol. Chem. 273(52): 34904-34910.
Pera et al. (2000) Human embryonic stem cells. J. Cell Science 113: 5-10.
GenBank Accession No. NM—005587—Homo sapiensMADS box transcription enhancer factor 2, polypeptide A (myocyte enhancer factor 2A) (MEF2A) mRNA.
GenBank Accession No. NM—005919—Homo sapiensMADS box transcription enhancer factor 2, polypeptide B (myocyte enhancer factor 2B) (MEF2B) mRNA.
GenBank Accession No. L08895—Homo sapiensMADS/MEF2-family transcription factor (MEF2C) mRNA, complete cds.
GenBank Accession No. NM—005920—Homo sapiensMADS box transcription enhancer factor 2, polypeptide D (myocyte enhancer factor 2D) (MEF2D) mRNA.
Arsenijevic and Weiss, “Insulin-Like Growth Factor-I Is a Differentiation Factor for Postmitotic CNS Stem Cell-Derived Neuronal Precursors: Distinct Actions from Those of Brain-Derived Neurotrophic Factor,”Journal of Neuroscience18:2118-2128 (1998).
Bain et al., “From Embryonal Carcinoma Cells to Neurons: The P19 Pathway,”Bioessays16:343-348 (1994).
Bain et al., “Embryonic Stem Cells Express Neuronal Properties in Vitro,”Developmental Biology168:342-357 (1995).
Bhatia et al., “A newly discovered class of human hematopoietic cells with SCID-repopulating activity,”Nature Medicine4:1038-1045 (1998).
Black et al., “Cooperative transcriptional activation by the neurogenic basic helix-loop-helix protein MASH1 and members of the myocyte enhancer factor-2 (MEF2) family,”J. Biol. Chem. 271:26659-26663 (1996).
Black and Olson, “Transcriptional Control of Muscle Development by Myocyte Enhancer Factor-2 (MEF2) Proteins,”Annual Rev. Cell Dev. Biol. 14:167-196 (1998).
Blaschke et al.,“Widespread programmed cell death in proliferative and postmitotic regions of the fetal cerebral cortex,”Development122:1165-1174 (1996).
Brand, “Molecules in Focus,”Int. J. Biochem. Cell Biol. 29:1467-1470 (1997).
Breitbart et al., “A fourth human MEF2 transcription factor, hMEF2D, is an early marker of the myogenic lineage,”Development118:1095-1106 (1993).
Brooker et al., “Endogenous IGF-1 Regulates the neuronal Differentiation of Adult Stem Cells,”J. Neurosci. Res.59:332-341 (2000).
Clem et al., “Prevention of apoptosis by a baculovirus gene during infection of insect cells,”Science254:1388-1390 (1991).
D'Amour and Gage, “New tools for human developmental biology,”Nature Biotechnology18:381-382 (2000).
Deveraux and Reed, “IAP family proteins—suppressors of apoptosis,”Genes&Development13:239-252 (1999).
Dunnett et al., “Neuronal cell transplantation for Parkinson's and Huntington's diseases,”British Medical Bulletin53:757-776 (1997).
Ekert et al., “Caspase Inhibitors,”Cell Death and Differentiation6:1081-1086 (1999).
English et al., “Contribution of the ERK5/MEK5 Patheway to Ras/Raf Signaling and Growth Conrol,”J. Biol. Chem. 274:31588-31592 (1999).
Fujimoto et al., “Identification of genes differentially expressed by putrescine in HepG2 hepatoblastoma cells,”Hepatology Research20:207-215 (2001).
Galpern, et al., “Xenotransplantation of porcine fetal ventral mesencephalon in rat model of Parkinson's disease: Functional recovery and graft morphology,”Exp. Neurol.140:1-13 (1996).
Garcia-Calvo et al., “Inhibition of Human Caspases by Peptide-based and Macromolecular Inhibitors,”J. Biol. Chem. 273:32608-32613 (1998).
Ghosh and Greenberg, “Distinct Roles for bFGF and NT-3 in the Regulation of Cortical Neurogenesis,”Neuron15:89-103 (1995).
Ghosh et al., “Requirement for BDNF in Activity-Dependent Survival of Cortical Neurons,”Science263:1618-1623 (1994).
Han et al., “Activation of the transcription factor MEF2C by the MAP kinase p38 in inflammation,”Nature386:296-299 (1997).
Jacks et al., “Effects of anRbmutation in the mouse,”Nature359:295-300 (1992).
Jan and Jan, “HLH proteins, fly neurogenesis, and vertebrate myogenesis,”Cell75:827-830 (1993).
Jo et al., “Differential display analysis of gene expression altered by ras oncogene,”Methods Enzymol. 332:233-244 (2001).
Johe et al., “Single factors direct the differentiation of stem cells from the fetal and adult central nervous system,”Genes and Development10:3129-3140 (1996).
Kato et al., “BMK1/ERK5 regulates serum-induced early gene expression through transcription factor MEF2C,”Embo J. 16:7054-7066 (1997).
Kato et al., “Bmk1/Erk5 is required for cell proliferation induced by epidermal growth factor,”Nature395:713-716 (1998).
Kaushal et al., “Activation of the myogenic lineage by MEF2A, a factor that induces and cooperates with MyoD,”Science266:1236-1240 (1994).
Khanna et al., “Metastasis-associated differences in gene expression in a murine model of osteosarcoma,”Cancer Res. 61:3750-3759 (2001).
Kuida et al., “Decreased apoptosis in the brain and premature lethality in CPP32-deficient mice,”Nature384:368-372 (1996).
Lee et al., “Gene expression profile of aging and its retardation by caloric restriction,”Science285:1390-1393 (1999).
Lee et al., “Potent and Selective Nonpeptide Inhibitors of Caspases 3 and 7 Inhibit Apoptosis and Maintain Cell Functionality,”J. Biol. Chem. 275:16007-16014 (2000).
Lee et al., “Myocyte-specific enhancer factor 2 and thyroid hormone receptor associate and synergistically activate the α-cardiac myosin heavy-chain gene,”
Lipton Stuart A.
Okamoto Shu-ichi
Falk Anne-Marie
Jones Day
Sanford-Burnham Medical Research Institute
LandOfFree
Methods of differentiating and protecting cells by... does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Methods of differentiating and protecting cells by..., we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Methods of differentiating and protecting cells by... will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-2670509