Methods of diagnosing multidrug resistant tuberculosis

Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving nucleic acid

Reexamination Certificate

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C435S091200, C536S023500, C536S024310, C536S024330

Reexamination Certificate

active

06905822

ABSTRACT:
The invention relates to the discovery that a putative gene ofMycobacterium tuberculosiswith no previously identified function is responsible for the ability of the bacterium to activate thioamide drugs. SinceM. tuberculosishas a low rate of synonymous mutations, all mutations in this gene, identified as Rv3854c and now termed “EtaA,” are expected to inhibit the ability of a bacterium with the mutation to activate a thioamide or thiocarbonyl drug. Thus, detecting a bacterium with a mutation in this gene indicates that the bacterium is resistant to treatment with thioamides.

REFERENCES:
Ahern et al. The Scientist, 1995 vol. 9 #15, p. 20.
Badcock et al, Jan. 14, 1997, Sanger centre Wellcome Genome campus.
Phillip et al., PNAS vol. 93, pp 3132-3137, Apr. 1996.
DeBarber et al. , PNAS vol. 97, No. 17, pp 9677-9682.
Boehringer Mannheim 1997 Biochemicals Catalog p. 95.

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