Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving oxidoreductase
Reexamination Certificate
2007-10-09
2007-10-09
Gitomer, Ralph (Department: 1657)
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving oxidoreductase
C435S244000
Reexamination Certificate
active
11235683
ABSTRACT:
The present invention provides a method for determining an antioxidant deficiency in a lymphocyte by determining the level of intracellular cysteine in the lymphocyte by measuring lymphocyte growth in a serum-free cell culture medium comprising cumene hydroperoxide and N-acetyl-L-cysteine (NAC), whereby any antioxidant deficiency correlates with lymphocyte growth in such a cell culture medium.
REFERENCES:
patent: 4499064 (1985-02-01), Shive
patent: 4927762 (1990-05-01), Darfler
patent: 5171885 (1992-12-01), Griffith
patent: 5290571 (1994-03-01), Bounous et al.
patent: 5326699 (1994-07-01), Torishima et al.
patent: 5405772 (1995-04-01), Ponting
patent: 5607974 (1997-03-01), Dr oge et al.
patent: 5985665 (1999-11-01), Crawford et al.
patent: 6262019 (2001-07-01), Keller et al.
patent: 6514955 (2003-02-01), Van Dyke
patent: 6709835 (2004-03-01), Crawford
patent: 6949382 (2005-09-01), Crawford
patent: 2002/0136763 (2002-09-01), Demopoulos et al.
Fidelus et al., “Modulation of intracellular glutathione concentrations alters lymphocyte activation and proliferation,”Exp. Cell. Res., 170:269-275, 1987.
Hamilos et al., “Lymphocyte proliferation in glutathione-depleted lymphocytes: direct relationship between glutathione availability and the proliferative response,”Immunopharmacology, 18:223-225, 1989.
Messina et al., “Effects of 2-mercaptoethanol and buthionine sulfoximine on cystine metabolism by and proliferation of mitogen-stimulated human and mouse lymphocytes,”Int. J. Immunopharmacology, 14:1221-1234, 1992.
Fulbright & Jaworski L.L.P.
Gitomer Ralph
Research Development Foundation
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