Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving antigen-antibody binding – specific binding protein...
Patent
1991-03-29
1994-06-14
Kepplinger, Esther L.
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving antigen-antibody binding, specific binding protein...
435 15, 435 723, 435 74, 530395, 436 64, G01N 3353, G01N 33544, C07K 1500, C12Q 148
Patent
active
053209435
ABSTRACT:
A method is provided for detecting T-cell dysfunctions. The method includes detecting an alteration in the level of a protein regulating synthesis of the hexasaccharide NeuNAc.alpha.2.fwdarw.3Gal.beta.1.fwdarw.3(NeuNAc.alpha.2.fwdarw.3Gal.beta .1.fwdarw.4GlcNAc.beta.1.fwdarw.6)GalNAc on leukosialin of T-cells from a subject suspected of having a T-cell dysfunction compared to resting T-cells from a normal individual. The protein regulating synthesis of the hexasaccharide can be core 2 GlcNAc transferase and can be detected by either a change in its amount or activity. Also provided is a method of detecting T-cell dysfunctions which includes detecting an alteration in the level of leukosialin having the hexasaccharide NeuNAc.alpha.2.fwdarw.3Gal.beta.1.fwdarw.3(NeuNAc.alpha.2.fwdarw.3Gal.beta .1.fwdarw.4GlcNAc.beta.1.fwdarw.6)GlcNAc on T-cells from a subject suspected of having a T-cell dysfunction compared to resting T-cells from a normal individual. Kits for detecting T-cell dysfunction are provided as well.
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Kepplinger Esther L.
La Jolla Cancer Research Foundation
Parsons Nancy J.
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