Methods of detecting hepatitis A virus

Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving virus or bacteriophage

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536 231, 536 2372, C12Q 170

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active

055166300

ABSTRACT:
Methods for producing HAV cDNA, products thereof, and uses thereof, are described. HAV cDNA is produced, for example, by reverse transcribing HAV RNA and subsequently inserting the HAV cDNA into bacterial plasmids by genetic-engineering techniques. Transformed bacteria are then cloned and cultured to produce replicated chimetic plasmids containing the HAV cDNA. Such HAV cDNA is useful in assaying for the presence of HAV and in the production of HAV antigen and in the production of antibodies against HAV.

REFERENCES:
Von der Helm et al. J. Virol. Methods. 3:37-43(1981) Cloning of Hepatitis A virus genome.
Max von Helm, (2nd International. Von Pett. Symposium, pp. 63-65) (1983).

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