Methods of detecting bovine herpesvirus 1 (BHV-1) in semen by nu

Details Methods of detecting bovine herpesvirus 1 (BHV-1) in semen by nu Methods of detecting bovine herpesvirus 1 (BHV-1) in semen by nu

1993-05-28

1996-08-13

Jones, W. Gary

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving nucleic acid

435 5, 435 911, 536 231, 536 243, 536 2432, 536 2433, 935 77, 935 78, C12Q 168, C12Q 170, C12P 1934

Patent

active

055455239

ABSTRACT:
The present invention relates to novel compositions comprising Bovine Herpesvirus-1 (BHV-1) specific oligonucleotides which are useful as nested primers to amplify sequences of the BHV-1 gIV gene during enzymatic nucleic acid amplification. The invention also provides a method for the detection of BHV-1 which may be present in a clinical specimen, particularly bovine semen, using the BHV-1 specific nested primers and enzymatic nucleic acid amplification. The present invention also relates to a BHV-1 specific oligonucleotide which can be used as a probe to facilitate detection of amplified products derived from BHV-1 gIV gene sequences.

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Israel et al., "Induction of a Mucosal Barrier to Bovine Herpesvirus 1 Replication in Cattle", Virology, Vol. 188, pp. 256-264 (1992).
Vilcek, "Detection of the bovine herpesvirus-1 (BHV-1) genome by PCR", Journal of Virological Methods, Vol. 41, (1993), pp. 245-248.

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