Methods of creating constructs useful for introducing...

Details Methods of creating constructs useful for introducing... Methods of creating constructs useful for introducing...

2005-09-13

2005-09-13

Leffers, Gerry (Department: 1636)

Chemistry: molecular biology and microbiology

Micro-organism, tissue cell culture or enzyme using process...

Preparing compound containing saccharide radical

C435S091100, C435S091200, C435S091500, C435S320100, C435S006120, C536S023100

Reexamination Certificate

active

06942995

ABSTRACT:
This invention provides novel nucleotide constructs. Also provided are methods for making DNA constructs useful for introducing sequences into and disrupting the function of a gene in a cell, particularly an embryonic stem cell.

REFERENCES:
patent: 5137814 (1992-08-01), Rashtchian et al.
patent: 5464764 (1995-11-01), Capecchi et al.
patent: 5487992 (1996-01-01), Capecchi et al.
patent: 5627059 (1997-05-01), Capecchi et al.
patent: 5631153 (1997-05-01), Capecchi et al.
patent: 5714667 (1998-02-01), Waterhouse et al.
patent: 6815185 (2004-11-01), Klein et al.
patent: WO 93/18175 (1993-09-01), None
patent: WO 94/06908 (1994-03-01), None
Aslanidis et al., 1990, Nucletic Acids Res., 18(20): 6069-6074, “Ligation-independent cloning of PCR products (LIC-PCR).”
Gubin et al., 1997, Biochem. Biophys. Res. Comm., 236: 347-350, “Long-Term, Stable Expression of Green Fluorescent Protein in Mammalian Cells.”
Randolph et al., 1996, Transgenic REs., 5(6): 413-420, “PCR-based Gene Targeting of the Inducible Nitric Oxide Synthase (NOS2) Locus in Murine ES Cells, a New and More Cost-effective Approach.”
Rizzuto et al., 1995, Current Biology, 5: 635-642, “Chimeric green fluorescent protein as a tool for visualizing subcellular organelles in living cells.”
Stratagene et al., Stratagene Catalog, 1997-1998, p35, “PCR Cloning and Plasmid Construction.”
Karaplis et al., Methods in Bone Research: 1189-1201, “Gene Targeting.”
US Dept. Health & Human Service, 1992, “Modified Vectors for ligation-independent cloning-using extended single-stranded complimentary tails on vectors and a foreign protein encoding sequence.”
Aslanidis et al., 1994, PCR Methods and Applications, 4: 172-177, “Minimal Length Requirement of the Single-stranded Tails for Ligation-independent Cloning of PCR Products.”
Ausubel F.M. et al., 1995, Current Protocols in Molecular Bio., (Table of Contents).
Freshney, R.I., Ed., 1987, IRL Press, “Animal Cell Culture: a practical approach.”
Jakoby, W.B., et al. Eds., 1979, Methods in Enzymology, vol. LVIII Cell Culture, Academic Press, Inc. (Table of Contents).
McPherson, M.J. et al., 1995, IRL Press, PCR 2: A Practical Approach (Table of Contents).
Munroe et al., 1995, Proc. Nat'l Acad. Sci., USSA 92(6): 2209-2213, “Systematic Screening of an Arrayed cDNA Library by PCR.”
Rashtchian, 1995, Current Opinion in Biotech., 6: 30-36, “Novel Methods for Cloning and Engineering Genes Using the Polymerase Chain Reaction.”
Sambrook et al., 1989, Molecular Cloning: A Laboratory Manual, 2nd Ed., Cold Spring Harbor Lab. Press (Table of Contents).
Sampath et al., 1997, Gene 190: 5-10“Versatile Vectors for Direct Cloning and Ligation-independent cloning of PCR-amplified fragments for surface display on filamentous bacteriophages.”
Yu et al., 1996, Methods Mol. Bio. 58: 335-339, “Use of Polymerase Chain Reaction to Screen Phage Libraries.”

Affiliated with

Also associated with

Rating

Methods of creating constructs useful for introducing... does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Methods of creating constructs useful for introducing..., we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Methods of creating constructs useful for introducing... will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFUS-PAI-O-3367361