Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving nucleic acid
Reexamination Certificate
2006-10-17
2006-10-17
Horlick, Kenneth R. (Department: 1637)
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving nucleic acid
C435S091200, C536S023100, C536S024300, C536S024330
Reexamination Certificate
active
07122310
ABSTRACT:
The present invention provides a novel method for forming a self-assembly substance of probes making it possible to shorten a reaction time required for formation of a self-assembly substance and also to increase regions of probes available for detection of a target gene by previously preparing a plurality of dimer-probes and increasing the types. This method comprises the steps of: providing plural groups, wherein each group includes a pair of dimer forming probes containing a pair of oligonucleotides, each oligonucleotide having three regions of a 3′ side region, a mid-region and a 5′ side region, in which the mid-regions of the oligonucleotides have base sequences complementary to each other, and the 3′ side regions and the 5′ side regions of the oligonucleotides have base sequences not complementary to each other; hybridizing a plurality of pairs of the dimer forming probes of the plural groups; and forming a double-stranded self-assembly substance by self-assembly of the oligonucleotides.
REFERENCES:
patent: 5175270 (1992-12-01), Nilsen et al.
patent: 5437977 (1995-08-01), Segev
patent: 5487973 (1996-01-01), Nilsen et al.
patent: 5605793 (1997-02-01), Stemmer
patent: 6261846 (2001-07-01), Usui
patent: 0 450 594 (1991-10-01), None
patent: 1 002 877 (2000-05-01), None
patent: 7-88000 (1995-04-01), None
patent: 10-500561 (1998-01-01), None
patent: 2000-201687 (2000-07-01), None
patent: 95/22625 (1995-08-01), None
patent: WO 99/06595 (1999-02-01), None
Ratilainen et al., “Hybridization of Peptide Nucleic Acid”,Biochemistry, vol. 37, pp. 12331-12342, 1998.
Prehn, “Hybridization of Uracil pentanucleotides with Poly-dA”,Acta biol. Med. Germ., vol. 31, pp. 885-887, 1973, in German with English translation thereof.
Hakii Chikako
Mitsuka Mari
Usui Mitsugu
Horlick Kenneth R.
Sanko Junyaku Co. Ltd.
Tung Joyce
Wenderoth , Lind & Ponack, L.L.P.
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