Methods of assaying differential expression

Details Methods of assaying differential expression Methods of assaying differential expression

1997-05-21

1999-11-30

Fredman, Jeffrey

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving nucleic acid

435 911, 435 912, 536 231, 536 243, 536 2431, 536 2433, C12Q 168, C12P 1934, C07H 2102, C07H 2104

Patent

active

059940760

ABSTRACT:
Methods and compositions are provided for analyzing differences in the RNA profiles between a plurality of different physiological samples. In the subject methods, a set of a representational number of distinct gene specific primers is used to generate labeled nucleic acids from each of the different physiological samples. The labeled nucleic acids are then compared to each other and differences in the RNA profiles are determined. The subject methods find use in methods of identifying differential gene expression.

REFERENCES:
patent: 4888274 (1989-12-01), Radding et al.
patent: 5162209 (1992-11-01), Scheele
patent: 5223414 (1993-06-01), Zarling et al.
patent: 5387510 (1995-02-01), Wu
patent: 5434047 (1995-07-01), Arnold, Jr.
patent: 5436327 (1995-07-01), Southern et al.
patent: 5449603 (1995-09-01), Nielson et al.
patent: 5468613 (1995-11-01), Erlich et al.
patent: 5487985 (1996-01-01), McClelland et al.
patent: 5503980 (1996-04-01), Cantor
patent: 5512462 (1996-04-01), Cheng
patent: 5547843 (1996-08-01), Studier
patent: 5580726 (1996-12-01), Villeponteau et al.
patent: 5599672 (1997-02-01), Liang et al.
Goodwin, Raymond G., et al., "Human Interleukin 7: Molecular Cloning and Growth Factor Activity on Human and Murine B-Lineage Cells," Proc. Natl. Acad. Sci. USA (Jan. 1989) vol. 86:302-306.
Leonard, Warren J., et al., "Molecular Cloning and Expression of cDNAs for the Human Interleukin-2 Receptor," Nature (Oct. 1984) vol. 311:626-631.
Nishi, Tatsunari et al., "Cloning and Expression of a Novel Variant of Human Interferon.UPSILON. cDNA," J. Biochem (1985) vol. 97:153-159.
Ehlers et al., "Differentiation of T cell lymphokine gene expression: the in vitro acquisition of T cell memory", J. Exp. Med. 173:25-36, Jan. 1991.
Goodwin et al, "Cloning of the human and murine interleukin 7 receptors: demonstration of a soluble form and homology to a new receptor superfamily", Cell 60:941-951, Mar. 1990.
Bauer, David et al., "Identification of Differentially expressed mRNA Species By An Improved Display Technique (DDRT-PCR)", Nucleic Acids Research (1993) vol. 21 No. 18; 4272-4280.
Chalifour, Lorraine et al., "A Method For Analysis of Gene Expression Patterns", Analytical Biochemistry (1994) vol. 216:299-304.
DeRisi, Joseph et al., "Use Of A cDNA Microarray To Analyse GeneExpression Patterns In Human Cancer", Nature Genetics vol. 14:457-460. (Dec. 1996).
Heller, Renu et al., "Discovery and Analysis of Inflammatory Disease-Related Genes Using cDNA Microarrays", Proc. Nat.l Acad. Sci. USA (1997) vol. 94:2150-2155.
Ikonomov, Ognian, et al., "Differential Display Protocol With Selected Primers That Preferentially Isolates mRNAs of Moderate-to-Low-Abundance in a Microscopic System", BioTechniques (1996) vol. 20 No.6; 1030-1042.
Liang, Peng et al., "Differential Display of Eukaryotic Messenger RNA by Means of the Polymerase Chain Reaction", Science (1992) vol. 257:967-971.
Liang, Peng et al., "Recent Advances in Differential Display", Current Opinion in Immunology (1995) vol. 7 :274-280.
Lockhart, David et al., "Expression Monitoring By Hybridization to High-Density Oligonucleotide Arrays", Nature Biotechnology (1996) vol. 14: 1675-1680.
Nguyen, Catherine et al., "Differential Gene Expression in the Murine Thymus Assayed by Quantitative Hybridization of Arrayed cDNA Clones", Genomics (1995) vol.29:207-216.
Pietu, Genevieve et al., "Novel Gene Transcripts Preferentially Expressed in Human Muscles Revealed by Quantitative Hybridization of a High Density cDNA Array", Genome Research (1996) vol.6:492-503.
Prashar, Yatindra et al., "Analysis of Differential Gene Expression by Display of 3' End Restriction Fragments of cDNAs", Proc. Nat.l Acad.Sci. USA (1996) vol.93: 659-663.
Schena, Mark et al., "Quantitative Monitoring of Gene Expression Patterns With a Complementary DNA Microarray", Science (1995) vol.270:467-470.
Schena, Mart et al., "Parallel Human Genome Analysis: Microarray-Based Expression Monitoring of 1000 Genes", Proc. Nat.l Acad. Sci. USA (1996) vol. 93:10614-10619.
Shalon, Dari et al., "A DNA Microarray System for Analyzing Complex DNA Samples Using Two-Color Fluorescent Probe Hybridization", Genome Research (1996) vol. 6: 639-645.
Sokolov, Boris et al., "A Rapid and Simple PCR-based Method For Isolation of cDNAs from Differentially Expressed Genes", Nucleic Acids Research (1994) vol. 22, No.19; 4009-4015.
Zhao, Nanding et al., "High-Density cDNA Filter Analysis: A Novel Approach for Large-Scale, Quantitative Analysis of Gene Expression", Gene (1995) vol. 156: 207-213.
"Atlas.TM. Human cDNA Expression Array I", Clontechniques Apr. 1997; 4-7.

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