Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving antigen-antibody binding – specific binding protein...
Patent
1988-02-09
1992-09-22
Kepplinger, Esther L.
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving antigen-antibody binding, specific binding protein...
204403, 436806, 436808, 435 25, 435810, 435817, 435 14, G01N 33536
Patent
active
051496305
ABSTRACT:
An electrochemical specific binding assay of a ligand (e.g., antigen, hapten or antibody) wherein at least one of the components is enzyme-labelled, and which includes the step of determining the extent to which the transfer of electrons between the enzyme substrate and an electrode, associated with the substrate reaction, is perturbed by complex formation or by displacement of any ligand complex relative to unbound enzyme-labelled component.
The electron transfer is aided by electron-transfer mediators which can accept electrons from the enzyme and donate them to the electrode or vice versa (e.g. ferrocene) or by electron-transfer promoters which retain the enzyme in close proximity with the electrode without themselves taking up a formal charge.
The electrochemical apparatus will typically comprise two or three electrodes, including one working electrode onto which components may advantageously be immobilized.
The use of direct electrochemical measurement of the enzyme label avoids the errors and inconvenience of the known indirect measurement techniques.
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Forrest Gordon C.
Hill Hugh A. O.
Rattle Simon J.
Robinson Grenville A.
Chin Christopher L.
Kepplinger Esther L.
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