Chemistry: molecular biology and microbiology – Enzyme – proenzyme; compositions thereof; process for... – Hydrolase
Patent
1996-02-13
1999-01-12
Wax, Robert A.
Chemistry: molecular biology and microbiology
Enzyme , proenzyme; compositions thereof; process for...
Hydrolase
435 6, 435191, 4352523, 43525233, 435 911, 536 231, 536 232, 536 234, 536 237, C12N 914, C12N 906
Patent
active
058587543
ABSTRACT:
A diagnostic method for detecting a base pair mismatch in a DNA duplex, comprising the steps of contacting at least one strand of a first DNA molecule with the complementary strand of a second DNA molecule under conditions such that base pairing occurs contacting a DNA duplex potentially containing a base pair mismatch with a mispair recognition protein under conditions suitable for the protein to form a specific complex only with the DNA duplex having a base pair mismatch, and not with a DNA duplex lacking a base pair mismatch, and detecting any complex as a measure of the presence of a base pair mismatch in the DNA duplex.
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Au Karin G.
Cooper Deani Lee
Lahue Robert S.
Modrich Paul L.
Su Shin-San
Duke University
Nashed Nashaat T.
Wax Robert A.
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