Methods of amplification using a thermostable DNA polymerase fro

Details Methods of amplification using a thermostable DNA polymerase fro Methods of amplification using a thermostable DNA polymerase fro

1996-05-24

2000-04-25

Fredman, Jeffrey

Chemistry: molecular biology and microbiology

Micro-organism, tissue cell culture or enzyme using process...

Preparing compound containing saccharide radical

435 6, 435 911, 435183, 530350, C12P 1934, C12Q 168, C07K 1500

Patent

active

060543019

ABSTRACT:
A nucleic acid amplifying enzyme having a short reaction time and high fidelity is provided. The enzyme of this invention is a thermostable DNA polymerase having a nucleic acid extension rate of at least 30 bases per second and a 3'-5' exonuclease activity. Also provided are a method and kit for amplifying nucleic acid.

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patent: 5491086 (1996-02-01), Gelfand
patent: 5500363 (1996-03-01), Comb
Mullis et al. Specific enzymatic amplification of DNA in vitro: the polymerase chain reaction, Cold Spring Harbor Symposia on Quantitative Biology, vol. 51, pt1, pp. 263-273, 1986.
A. Skerra, Nucl. Acids Res., Abstract, "Two Thermostable DNA Polymerase with, Proofreading Activity--Vent DNA Polymerase and Pfu Polymerase" 20:14, pp. 3551-3554, (1992).
Kitabayashi, M., FASEB Journal, 10:6, p. 1423, Apr. 30, 1996, "KOD DNA-Polymerase-Application for fast and accurate PCR".
Kakihara, H., unpublished, "Cloning and Analysis of the DNA polymerase gene from a new hyperthermophilic archaeon, Pyrococcus sp. strain KOD1" undated.
Database EMBL, DNA-Sequence Data Library, Hiedelberg, BRD Creation AC D29671, Apr. 23, 1994, XP002017309.
Hirofumi Kakihara et al, Abstract No. 469, p. 168, 1993 General Meeting of Japan Association of Biotechnology, published Nov. 10 1993, presented Dec. 08 1993.
Motomu Nishioka et al, Abstract No. 354, p. 95, 1994 General Meeting of Japan Association of Biotechnology, published Oct. 10 1994, presented Nov. 29 1994.
Hirofumi Kakihara et al, Abstract No. 2Za9, Nippon Nogeikagaku Kaishi, vol. 68, No. 3, p. 146, published Mar. 05 1994.
Masaaki Morikawa et al, Applied and Environmental Microbiology, vol. 60, No. 12, Dec. 1994, pp. 4559-4566.

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