Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or...
Reexamination Certificate
2005-07-14
2010-02-09
Haq, Shafiqul (Department: 1641)
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
C435S007100, C436S514000, C436S053000, C436S823000
Reexamination Certificate
active
07659053
ABSTRACT:
The present invention provides a series of methods, compositions, and articles for altering a property of a surface (for example, the cytophilicity and/or the hydrophilicity), by exposing at least a portion of the surface to a non-chemical, force-creating field and/or force, such as an electric field. The field/force may be created by any suitable technique. For instance, the field can be created by applying a voltage across the surface, by electrical induction, etc. In certain embodiments, the surface includes molecules attached thereto that can be detached when exposed to non-chemical, force-creating fields and/or forces, thereby altering the chemical composition of at least a portion of the surface. In one set of embodiments, the molecules attached to the surface may include molecules forming a self-assembled monolayer on the surface. In some embodiments, the molecules attached to the surface may include thiol moieties (e.g., as in an alkanethiol), by which the molecule can become attached to the surface. In certain cases, the molecules may be terminated at the unattached end with one or more hydrophilic groups, for example, unmodified ethylene glycol moieties. In some cases, the molecules attached to the surface may include one or more moieties that can bind to various entities such as proteins, peptides, nucleic acids, drugs, cells, etc. In certain embodiments, the techniques are used to enable novel assays for cell motility and/or spreading and screening tests for determining drugs and/or treatments effective in increasing or decreasing cell shape changes and/or motility on surfaces.
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Ferrigno Rosaria
Jiang Xingyu
Whitesides George M.
Haq Shafiqul
President and Fellows of Harvard College
Wolf Greenfield & Sacks P.C.
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