Methods of affecting intracellular phosphorylation of tyrosine u

Drug – bio-affecting and body treating compositions – Designated organic active ingredient containing – Carbohydrate doai

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514 43, A01N 4304, A61K 31705

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active

060309555

ABSTRACT:
The present invention provides methods of stimulating or inhibiting intracellular phosphorylation of tyrosine by a heparin-binding receptor which comprises contacting a cell having on its surface the heparin-binding receptor with a phosphorothioate oligonucleotide moiety of suitable length and base composition, such phosphorothioate oligonucleotide moiety being present in an effective amount. The invention further provides a method of inhibiting the formation of blood vessels in a subject which comprises administering to the subject an amount of a phosphorothioate oligonucleotide moiety of suitable length and base composition. The invention also provides a method of inhibiting proliferation of cells having a malignant phenotype in a subject which comprises administering to the subject an amount of a phosphorothioate oligonucleotide moiety of suitable length and base composition.

REFERENCES:
Abstract to Sakano et al. of JP 94-320712, Dec. 22, 1994.
Stedman's Medical Dictionary, 24th Edition, pp. 777 and 1254, Feb. 1983.
Guvakova, M.A., et al., "Phosphorothioate Oligodeoxynucleotides Bind to Basic Fibroblast Growth Factor, Inhibit Its Binding to Cell Surface Receptors, and Remove It from Low Affinity Binding Sites on Extracellular Matrix." J. Biol. Chem. 270: 2620-2627 (1995).
Khalad, Z., et al., "Effects of Suramin-related and Other Clinically Therapeutic Polyanions on Protein Kinase C Activity.sup.1." Clin. Cancer Res. 1: 113-122 (1995).
Millauer, B., et al., "Glioblastoma growth inhibited in vivo by a dominant-negative F1k-1 mutant." Nature (Lond) 367: 576-579 (1994).
Yakubov, L., et al., "Oligodeoxynucleotides Interact with Recombinant CD4 at Multiple Sites*." J. Biol. Chem. 268: 18818-18823 (1993).

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