Methods, kits, and reactive supports for 3' labeling of oligonuc

Details Methods, kits, and reactive supports for 3' labeling of oligonuc Methods, kits, and reactive supports for 3' labeling of oligonuc

1990-12-20

1994-03-01

Schwartz, Richard A.

Organic compounds -- part of the class 532-570 series

Organic compounds

Carbohydrates or derivatives

501 33, 524 1, 435 6, C12Q 168, C03C 1200, C07H 1700

Patent

active

052909255

ABSTRACT:
In a first aspect, the invention involves a reactive support useful for automated synthesis of oligonucleotides. The reactive support comprises a label moiety (e.g. hapten) covalently bonded via a stable bond to a trifunctional spacer. The labeled trifunctional spacer complex is covalently bonded to a solid support via a cleavable bond. One arm of the trifunctional spacer attaches the solid phase; another arm attaches the label; while the third arm provides a hydroxyl group useful for synthesizing a labeled oligonucleotide. Upon synthesis, the cleavable bond is broken, yielding the labeled oligonucleotide. Methods for labeling oligonucleotides and useful kits are also described.

REFERENCES:
Humphrey et al. (1970), Immunology for Students of Medicine, 3rd edition, (Blackwell Scientific Publications, Oxford), p. 715.
Goodchild, Bioconjugate Chemistry, 1(3):165-187 (1990).
Kempe, et al, Nucleic Acids Research, 13(1):45-57 (1985).
Zuckermann, et al., Nucleic Acids Research, 15(13):5305-5321 (1987).
Nelson, et al., Nucleic Acids Research, 17(18):7187-7194 (1989).
Usman, et al., Tetrahedron Letters, 29(38 ):4831-4834 (1988).

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