Methods, kits, and compositions for detecting enzyme activity

Details Methods, kits, and compositions for detecting enzyme activity Methods, kits, and compositions for detecting enzyme activity

2011-04-19

2011-04-19

Carlson, Karen Cochrane (Department: 1657)

Chemistry: molecular biology and microbiology

Apparatus

Including measuring or testing

Reexamination Certificate

active

07927864

ABSTRACT:
The inventive subject matter relates to methods, kits, and compositions for detecting enzyme activity in a biological sample. In particular, the inventive subject matter relates to methods, kits, and compositions for detecting von Willebrand factor degrading enzyme activity in a biological sample.

REFERENCES:
patent: 2007/0065895 (2007-03-01), Miyata et al.
Kokame et al. “FRETS-VWF73, a first fluorogenic substrate for ADAMTS13 assay”, British Journal of Haematology, 2005, 129:93-100.
Kruger et al. “An economical and preparative orthogonal solid phase synthesis of fluorescein and rhodamine derivatized peptides: FRET substrates for theStaphylococcus aureussortase SrtA transpeptidase reation”, Chem, Commun. 2002:2092-2093.
Kokame et al. British Journal of Haematology 2005;129:93-100.
Zhang Biochem & Biophy Res Communication 2004, 323:674-678.
Zanardelli, et al., ADAMTS13 substrate recognition of von Willebrand factor A2 domain. J Biol Chem. Jan. 20, 2006;281 (3): 1555-63. Epub Oct. 12, 2005.
Sutherland, et al., Molecular modeling of the von Willebrand factor A2 Domain and the effects of associated type 2A von Willebrand disease mutations. J Mol Model. Aug. 2004;10(4):259-70. Epub Aug. 3, 2004.
Hassenpflug, et al., Impact of mutations in the von Willebrand factor A2 domain on ADAMTS13-dependent proteolysis. Blood. Mar. 15, 2006;107(6):2339-45. Epub Dec. 1, 2005.
Kokame, et al., VWF73, a region from D1596 to R1668 of von Willebrand factor, provides a minimal substrate for ADAMTS-13. Blood. Jan. 15, 2004;103(2):607-12. Epub Sep. 25, 2003.
Jenkins, et al., Molecular modeling of ligand and mutation sites of the type A domains of human von Willebrand factor and their relevance to von Willebrand's disease. Blood. Mar. 15, 1998;91(6):2032-44.
Dong, et al., ADAMTS-13 metalloprotease interacts with the endothelial cell-derived ultra-large von Willebrand factor. J Biol Chem. Aug. 8, 2003;278(32):29633-9. Epub May 29, 2003.
Anderson, et al., Zinc and calcium ions cooperatively modulate ADAMTS13 activity. J Biol Chem. Jan. 13, 2006;281 (2):850-7. Epub Nov. 11, 2005.
Majerus, et al., Binding of ADAMTS13 to von Willebrand factor. J Biol Chem. Jun. 10, 2005;280(23):21773-8. Epub Apr. 11, 2005.
Sheffield University VWF Information Homepage, including links to von Willebrand Factor mutations and polymorphisms associated with von Willebrand Diseases; Type 2A mutations: see http://www.ragtimedesign.com/vwf/mutation/mutationtableresults.php; Type 2A polymorphisms: http://www.ragtimedesign.com/vwf/polymorphism/polytableresults.php.

Affiliated with

Also associated with

Rating

Methods, kits, and compositions for detecting enzyme activity does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Methods, kits, and compositions for detecting enzyme activity, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Methods, kits, and compositions for detecting enzyme activity will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFUS-PAI-O-2666624