Methods, hybridomas, monoclonal antibodies and sensitized cells

Details Methods, hybridomas, monoclonal antibodies and sensitized cells Methods, hybridomas, monoclonal antibodies and sensitized cells

1988-08-17

1992-01-07

Wallell, Frederick E.

Chemistry: analytical and immunological testing

Involving an insoluble carrier for immobilizing immunochemicals

Carrier is a biological cell or cell fragment

436518, 436501, G01N 33556, G01N 33543, G01N 33566

Patent

active

050791737

ABSTRACT:
This invention provides anti-HBs monoclonal antibodies HBs 8Cl, HBs 18E9 and HBs 22B7, an anti-human IgM monoclonal antibody HIgM 10C9 and an anti-mumps virus monoclonal antibody MPV 10G3 capable of inhibiting nonspecific reaction in the reverse passive agglutination, hybridomas producing them, a method for sensitizing fixed hemocytes with anti-HBs monoclonal antibodies, a fixed hemocyte sensitized with them by the said method, a method for inhibiting the said nonspecific reaction by utilizing the said monoclonal antibodies and an assay for HBs antigen by appropriately combining the above.

REFERENCES:
patent: 4271145 (1981-06-01), Wands et al.
patent: 4680274 (1987-07-01), Sakai
Roitt et al., "Immunology" Gower Publishing Co. (1977) p. 25.3.
Steinitz & Tamir, "The Coating of . . . ", J. Immuno. Methods, 76 (1985) 27-38.
Sasaki et al., "Passive Hemaggulination", J. Immuno. Methods 22:327-337 (1978).

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