Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving nucleic acid
Reexamination Certificate
2006-07-25
2006-07-25
Horlick, Kenneth R. (Department: 1637)
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving nucleic acid
C435S091200
Reexamination Certificate
active
07081339
ABSTRACT:
The invention relates to methods of monitoring the amplification of one or more nucleic acid sequences of interest. More particularly, the invention relates to methods of monitoring the amplification of sequences of interest in real time. The methods disclosed herein provide methods for monitoring the amplification of one sequence or two or more sequences from a single sample, as well as methods for monitoring the amplification of one or more than one sequence from two or more samples. The monitoring methods of the invention permit improved determination of the abundance of one or more target nucleic acids, especially target RNA species, in one or more original samples.
REFERENCES:
patent: 5691146 (1997-11-01), Mayrand
patent: 6221600 (2001-04-01), MacLeod
patent: 6482615 (2002-11-01), Tal et al.
patent: 6495326 (2002-12-01), Kurane et al.
patent: 6531282 (2003-03-01), Dau et al.
patent: 2002/0028452 (2002-03-01), Wittwer et al.
Sanchez-Vega et al., Laboratory Investigation 82(1), 345A (Jan. 2002).
Sanchez-Vega et al., J. Mol. Diagnostics 4(4), 223-229 (Nov. 2002).
International Search Report of International Application No. PCT/US03/37420.
Holland, et al., “Detection of specific polymerase chain reaction product by utilizing the 5'-3'exonuclease activity ofThermus aquaticusDNA polymerase”, 1991, Proc. Natl. Acad. Sci. USA 88:7276-7280.
Borson, et al., “Direct Quantitation of RNA Transcripts by Competitive Single-Tube RT-PCR and Capillary Electrophoresis”, 1998, Biotechniques 25: 130-7.
Rajcevic, et al., “Assessment of differential expression of oncogenes in gastric adenocarcinoma by fluorescent multiplex RT-PCR assay”, 2001, Pflugers Arch., Eur. J. Physiol. 442 (6 Suppl 1): R190-2.
Odin et al., “Rapid method for relative gene expression determination in human tissues using automated capillary gel electrophoresis and multicolor detection”, 1999, J. Chromatogr. B, 734:47-53.
George, et al., “Capillary electrophoresis methodology for identification of cancer related gene expression patterns of fluorescent differential display polymerase chain reaction”, 1997, J. Chromatogr. B., 695: 93-102.
Omori, et al., “Comparative PCR: A Simple and Sensitive Method for Quantifying Low-Abundance mRNA Species”, 2000, Genomics 67: 140-5.
O'Dorschner, et al., “Diagnosis of Five Spinocerebellar Ataxia Disorders by Multiplex Amplification and Capillary Electrophoresis”, Journal of Molecular Diagnostics (2002), V. 4, No. 2, pp. 108-113.
Erdman, et al., “GeneScan Reverse Transcription-PCR Assay for Detection of Six Common Respiratory Viruses in Young Children Hospitalized with Acute Respiratory Illness”, Journal of Clinical Microbiology (2003), V. 41, No. 9, pp. 4298-4303.
Li, et al., “Quantitative Polymerase Chain Reaction Using Capillary Electrophoresis with Laser-Induced Fluorescence Detection: Analysis of Duck Hepatitis B”, Anal Bioannal Chem (2002), V. 374, pp. 269-273.
Martin, et al., “Automated Ribosomal DNA Fingerprinting by Capillary Electrophoresis of PCR Products”, Analytical Biochemistry (1993), V. 214, pp. 182-189.
Odin, et al., “Rapid Method for Relative Gene Expression Determination in Human Tissues Using Automated Capillary Gel Electrophoresis and Multicolor Detection”, Journal of Chromatography (1999), V. 734, pp. 47-53.
Lawrence, et al., “Evaluation of Semiautomated Multiplex PCR Assay for Determination ofStreptococcus pneumoniaeSerotypes and Serogroups” Journal of Clinical Microbiology (2003), V. 41, No. 2, pp. 601-607.
Edwards Angell Palmer & Dodge
FitzGerald Mark J.
Horlick Kenneth R.
Primera BioSystems, Inc.
Williams Kathleen M.
LandOfFree
Methods for variation detection does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Methods for variation detection, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Methods for variation detection will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-3559618