Methods for using mutant RNA polymerases with reduced discrimina

Details Methods for using mutant RNA polymerases with reduced discrimina Methods for using mutant RNA polymerases with reduced discrimina

1996-09-13

1998-12-15

Robinson, Douglas W.

Chemistry: molecular biology and microbiology

Micro-organism, tissue cell culture or enzyme using process...

Preparing compound containing saccharide radical

435 912, 536 243, 536 245, C07H 2104, C12P 1934

Patent

active

058495460

ABSTRACT:
A method for synthesizing a nucleic acid molecule comprising at least one non-canonical nucleoside triphosphate using a mutant polymerase having a reduced discrimination between canonical and non-canonical substrates is disclosed. The method comprises incubating a template nucleic acid in a reaction mixture comprising the mutant nucleic acid polymerase and the appropriate canonical and non-canonical nucleoside triphosphates which are desired substrates for the mutant nucleic acid polymerase. The present invention is also a method of determining the sequence of a nucleic acid molecule using the mutant polymerase to create a nucleic acid molecule comprising at least one non-canonical nucleoside triphosphate.

REFERENCES:
patent: 4683195 (1987-07-01), Mullis et al.
Kostyuk DA, et al. "Mutants of T7 RNA polymerase that are able to synthesize both RNA and DNA." FEBS Letters 369: 165-168, Aug. 7, 1995.
Cazenave C, et al. "RNA template-directed RNA synthesis by T7 RNA polymerase." PNAS 91: 6972-6976, 1994.
Uhlmann E, et al. "Antisense oligonucleotides: A new therapeutic principle." Chem. Rev. 90: 543-593, 1990.
Wolfgang A, et al. "Kinetic characterization of ribonuclease-resistant 2'-modified hammerhead ribozymes." Science 253: 314-317, 1991.
"Probe Amplifier System Based on Chimeric Cycling Oligonucleotides," Biotechniques 9(2) :142-146, 1990.
W.M. Barnes, "DNA Sequencing by Partial Ribosubstitution," J. Mol. Biol. 119:83-99, 1978.
E.T. Butler and M.J. Chamberlin, "Bacteriophage SP6-specific RNA Polymerase," J. Biol. Chem. 257(10) :5772-5778, 1982.
D.H. Jones and B.H. Howard, "A Rapid Method for Recombination and Site-Specific Mutagenesis by Placing Homologous Ends on DNA Using Polymerase Chain Reaction," Biotechniques 10(1) :62-66, 1991.
G.A. Kassavetis, et al., "Bacteriophage SP6-specific RNA Polymerase," J. Biol. Chem. 257(10) 5779-5788, 1982.
H. Kotani, et al., "Nucleotide sequence and expression of the cloned gene of bacteriophage SP6 RNA polymerase," Nucl. Acids Res. 15(6) :2653-2664, 1987.
R. Sousa and R. Padilla, "A mutant T7 RNA polymerase as a DNA polymerase," EMBO J. 14(18) :4609-4621, 1995.

Affiliated with

Also associated with

Rating

Methods for using mutant RNA polymerases with reduced discrimina does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Methods for using mutant RNA polymerases with reduced discrimina, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Methods for using mutant RNA polymerases with reduced discrimina will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFUS-PAI-O-1456959