Chemistry: molecular biology and microbiology – Treatment of micro-organisms or enzymes with electrical or... – Modification of viruses
Patent
1997-12-17
1999-07-06
Degen, Nancy
Chemistry: molecular biology and microbiology
Treatment of micro-organisms or enzymes with electrical or...
Modification of viruses
435 691, 4352523, 43525231, 43525233, 4353201, C12N 1563, C12N 1570, C12N 1574, C12N 121
Patent
active
059196785
ABSTRACT:
A method for the inactivation of a gene in a bacterium or for the introduction of a heterologous gene into a bacterium comprises a) introducing, by transformation or conjugation, in the bacterium a bacterial vector plasmid comprising a marker gene capable of being expressed in a bacterial host strain and an effective replication system which is temperature sensitive at and above a temperature compatible with the viability of the host strain; b) culturing the bacterium on a selective medium at a temperature below the temperature of inhibition to form a culture; c) raising the temperature of the culture to a temperature above the temperature of inhibition; and d) recovering the surviving bacteria after several multiplication cycles.
REFERENCES:
patent: 4711845 (1987-12-01), Gelfand et al.
Applied and Environmental Microbiology; vol. 57, No. 2, 1991, Washington DC, pp. 539-548. Feiertag J M; Petzel J P; Pasalodos E; Baldwin K A; McKay L L "Thermosensitive Plasmid Replication Temperature-Sensitive Host Growth and Chromosomal Plasmid Integration Conferred by Lactococcus-Lactis-SSP-Cremoris Lactose Plasmids in Lactococcus-Lactis-SSP-Lactis" see the whole document. Relevant to Claims 1-4, 19-24.
Journal of Bacteriology; vol. 172, No. 8, 1990, Baltimore, U.S., pp. 4543-4584. Sozhammannan S; Dabert P; Moretto V; Ehrlich S D; Gruss A "Plus-Origin Mapping of Single-Stranded DNA Plasmid P-E-194 and Nick Site Homologies With Other Plasmids" cited in the application. See the whole document. Relevant to Claims 1, 2, 19-24.
Biological Abstracts; vol. 87 Philadelphia, PA, U.S., abstracts No. 047423. Alonso J C; Stiege C A; Tailor R H; Viret J-F "Functional Analysis of the DNA-TS Mutants of Bacillus-Subtilis Plasmid PUB110 Replication As A Model System"; see abstract & Mol Gen Genet 214 (3). 1988. 482-489. Relevant to Claims 1, 9-11.
Biological Abstracts; vol. 92, Philadelphia, PA, U.S., abstract No. 075453. Leenhouts K J; Kok J; Venema G "Replacement Recombination In Lactococcus-Lactis"; see abstract & J. Bacteriol 173 (15). 1991. 4794-4798. Relevant to Claims 5,8.
Biological Abstracts; vol. 88, Philadelphia, PA, U.S., abstract No. 107483. Priebe S D; Lacks S A "Region Of The Streptococcal Plasmid PMV158 Required For Conjugative Mobilization"; see abstract & J. Bacteriol 171 (9). 1989. 4778-4784. Relevant to Claim 15.
Journal of Bacteriology; vol. 174, No. 17, 1992, Baltimore U.S., pp. 5633-5638. Maguin E; Duwat P; Hege T; Ehrlich D; Gruss A "New Thermosensitive Plasmid For A Gram-Positive Bacteria"; see the whole document. Relevant to Claims 1-24.
Alonso et al. "Functional Analysis of the dua(Ts) Mutants of Bacillus Subtilic" Mol Gen Genet vol. 214, pp. 482-489, 1988.
Danilevich et al. "Isolation & Characterization of a Temperature-Sensitive Plasmid . . . " Mol. Biol. 18(4), 1111-1120, 1984. Abstract only.
Urlapova et al. "Temperature-Sensitive Mutants of the Plasmid RP-1" Genetuka (15) (13), 433-443, 1979. Abstract only.
Leenhouts et al. "Nucleotide Sequence & Characterization of the Broad Host Range Lactococcol Plasmid pWVO1" 26, 55-66, 1991.
Gruss Alexandra
Maguin Emmanuelle
Degen Nancy
Institut National de la Recherche Agronomique
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