Methods for use of MPL ligands with primitive human stem cells

Details Methods for use of MPL ligands with primitive human stem cells Methods for use of MPL ligands with primitive human stem cells

2005-07-01

2008-08-26

Paras, Jr., Peter (Department: 1632)

Chemistry: molecular biology and microbiology

Animal cell, per se ; composition thereof; process of...

C435S366000, C435S384000, C435S395000

Reexamination Certificate

active

07416887

ABSTRACT:
Myeloproliferative leukemia receptor (mpl) ligands, such as thrombopoietin, act on a primitive subpopulation of human stem cells having the characteristics of self-renewal and ability to give rise to all hematopoietic cell lineages. Thrombopoietin supports both megakaryocytic differentiation and primitive progenitor cell expansion of CD34+and CD34+sub-populations (CD34+Lin−, CD34+Thy-1+Lin−, and CD34+Lin−Rh123−lo). Thrombopoietin also stimulated quiescent human stem cells to begin cycling. Thus, mpl ligands are useful for expanding primitive stem cells for restoration of hematopoietic capabilities and for providing modified human stem cells for gene therapy applications.

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