Methods for use of mismatch repair systems for the detection and

Details Methods for use of mismatch repair systems for the detection and Methods for use of mismatch repair systems for the detection and

1996-12-13

1999-07-13

Degen, Nancy

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving nucleic acid

435 911, 435 912, 435183, 435193, 435810, 536 231, 536 243, C12Q 168, C07H 2104, C12N 910

Patent

active

059225394

ABSTRACT:
Method for removing DNA molecules containing one or more polymerase-generated mutations in a population of enzymatically amplified DNA duplexes comprising the steps of denaturing and reannealing the population of DNA duplexes, contacting the reannealled DNA duplexes with a mismatch repair system such that each strand is cleaved in DNA duplexes containing a base pair mismatch, and separating the cleaved DNA duplexes from uncleaved DNA duplexes.

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