Methods for transforming plants

Details Methods for transforming plants Methods for transforming plants

2007-08-14

2007-08-14

Fox, David T. (Department: 1638)

Multicellular living organisms and unmodified parts thereof and

Method of introducing a polynucleotide molecule into or...

Involving electroporation

C800S260000, C800S278000, C800S294000, C800S312000, C800S313000, C435S189000, C536S023200, C536S023600

Reexamination Certificate

active

09206852

ABSTRACT:
Methods for the transformation of plants are provided. The methods of the present invention comprise the steps of a) contacting the meristematic tissue of a plant and an area of the plant below the contacted meristematic tissue to a power source, wherein the area below the meristematic tissue is contacted to a positive lead and the meristematic tissue is contacted with a DNA-containing medium which in turn is contacted with a negative lead, and b) applying low amperage current, thus causing the DNA to migrate from the DNA-containing medium into the cells of the meristematic tissue. The transformed plants may be grown to maturity. The mature plants may then self-pollinate and the seed from the plant harvested. Both the parent plant, seed from the parent plant, progeny plants and progeny seeds contain the introduced DNA.

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