Methods for transforming immature maize embryos

Details Methods for transforming immature maize embryos Methods for transforming immature maize embryos

2006-06-06

2006-06-06

Kallis, Russell P. (Department: 1638)

Multicellular living organisms and unmodified parts thereof and

Method of introducing a polynucleotide molecule into or...

Involving particle-mediated transfecion

C800S298000, C800S300100, C800S320100

Reexamination Certificate

active

07057089

ABSTRACT:
Methods are provided for transforming freshly isolated, immature maize embryos and for producing transgenic maize plants. The methods comprise obtaining immature embryos from a maize plant, contacting the embryos with an auxin-depleted or phytohormone-depleted transformation support medium and introducing a nucleotide construct into cells from the embryos prior to subjecting the embryos to conditions which promote embryogenic-tissue formation. The methods additionally comprise identifying or selecting transformed cells and regenerating such cells into transformed maize plants.

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