Methods for the rapid analysis of the reticulocytes

Details Methods for the rapid analysis of the reticulocytes Methods for the rapid analysis of the reticulocytes

1996-12-20

1998-06-30

McMahon, Timothy

Chemistry: analytical and immunological testing

Biological cellular material tested

436 8, 436 10, 436 17, 436 18, 436800, 435 6, 435 724, 435 725, G01N 3348

Patent

active

057732990

ABSTRACT:
A method and reagent for the simultaneous or independent enumeration of reticulocytes in a whole blood sample, without the need to separately incubate the sample and reagent. The reagent contains a reticulocyte staining amount of an unsymmetrical cyanine dye, from about 40 mM to about 60 mM of a buffer selected from the group consisting of imidazole, Tris and Bis-Tris and a dye stabilizing amount of a non-ionic surfactant cholamide and a polyoxypropylene-polyoxyethylene block copolymer. The reagent has a pH from about 6.8 to about 7.2 and an osmolarity adjusted to about 280 to about 310 mosm/l with a mono-, or di-, valent alkali salt selected from the group consisting of NaCl, KCl, LiCl, CaCl.sub.2, MgCl.sub.2 and ZnCl.sub.2. The method utilizes the reagent in a no incubation process that also allows for the simultaneous determination of CBC as well as reticulocyte counts and maturity indices.

REFERENCES:
patent: 5436134 (1995-07-01), Haugland et al.

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