Drug – bio-affecting and body treating compositions – Antigen – epitope – or other immunospecific immunoeffector – Conjugate or complex
Patent
1997-04-03
1999-10-19
Wortman, Donna C.
Drug, bio-affecting and body treating compositions
Antigen, epitope, or other immunospecific immunoeffector
Conjugate or complex
530403, 514 54, 514 59, A61K 39385
Patent
active
059685158
ABSTRACT:
The invention comprises a low temperature method for the in situ derivatization and coupling of thermally and hydrolytically labile organic compounds such as spectinomycin to immunogenic carrier substances. The compound coupled to the immunogenic carrier is utilized to produce antibodies selective for the organic compound. Such methods allow for the quantitative analysis of the target organic compound and may be competitive or non-competitive immunoassays in which the dose response is directly or indirectly proportional to the concentration of spectinomycin-related substances. Such assays may be used to detect the presence of or to quantitate the amount of the organic compound in a complex and varied immunological background such as a sample of a biomass material. Detection of organic compound in the sample occurs through binding to a specific antibody. Amplification of that antibody-antigen response to increase the level of detection can be accomplished with a variety of markers producing either a fluorescent, ultraviolet, visible, chemiluminescent, gravimetric, amperometric, voltametric, or radioactive response.
REFERENCES:
Brunswick et al., Specific Radiommunoassay of Amitriptyline and Nortriptyline. British Journal of Clinical Pharmacology 7:343-348, 1979.
Campbell et al., Detection and Quantitation of Chloramphenicol by Competitive Enzyme-Linked Immunoassay, Antimicrobial Agents and Chemotherapy 25(2): 205-211, 1984.
Erlanger, The Preparation of Antigenic Hapten-Carrier Conjugates: A Survey, Methods in Enzymology, vol. 70, pp. 85-104, 1980.
Tanaka et al., Easy Enzyme-Llnked Immunosorbent Assay for Spectinomycin in Chicken Plalsma, Journal of AOAC International 79(2):426-430, 1996.
Thaco Research, Ltd.
Wortman Donna C.
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