Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving antigen-antibody binding – specific binding protein...
Patent
1996-06-28
1998-07-14
Knode, Marian C.
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving antigen-antibody binding, specific binding protein...
436518, 436543, 530403, G01N 33531
Patent
active
057802432
ABSTRACT:
The invention comprises a low temperature method for the in situ derivatization and coupling of thermally and hydrolytically labile organic compounds such as spectinomycin to immunogenic carrier substances. The compound coupled to the immunogenic carrier is utilized to produce antibodies selective for the organic compound. Such methods allow for the quantitative analysis of the target organic compound and may be competitive or non-competitive immunoassays in which the dose response is directly or indirectly proportional to the concentration of spectinomycin-related substances. Such assays may be used to detect the presence of or to quantitate the amount of the organic compound in a complex and varied immunological background such as a sample of a biomass material. Detection of organic compound in the sample occurs through binding to a specific antibody. Amplification of that antibody-antigen response to increase the level of detection can be accomplished with a variety of markers producing either a fluorescent, ultraviolet, visible, chemiluminescent, gravimetric, amperometric, voltametric, or radioactive response.
REFERENCES:
Campbell et al., Detection and Quantitation of Chloramphenicol by Competitive Enzyme-Linked Immunoassay, Antimicrobial Agents and Chemotherapy 25(2):205-211, 1984.
Tanaka et al., Easy Enzyme-Linked Immunosorbent Assay for Spectinomycin in Chicken Plasma, Journal of AOAC International 79(2):426-430, Mar.-Apr. 1996.
The Merck Index, Eleventh Edition, Budavari et al., Eds. Merck & Co., Inc. Rahway, N.J., 1989, p. 1341 and p. 1378.
Erlanger, B., The Preparation of Antigen-Hapten-Carrier Conjugates: A Survey. Methods in Enzymology 70:85-104, 1980.
Maggio, Ed. Enzyme Immunoassay. CRC Press, Boca Raton, 1980, pp. 167-179.
Knode Marian C.
Remenick James
Thaco Research, Ltd.
Wortman Donna C.
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