Methods for the production of stably-transformed, fertile...

Details Methods for the production of stably-transformed, fertile... Methods for the production of stably-transformed, fertile...

2006-04-11

2006-04-11

Collins, Cynthia (Department: 1638)

Multicellular living organisms and unmodified parts thereof and

Method of introducing a polynucleotide molecule into or...

Via agrobacterium

C435S469000, C435S430100

Reexamination Certificate

active

07026528

ABSTRACT:
Disclosed are processes for producing stably transformed fertile wheat a system of transforming wheat viaAgrobacterium. This invention provides methods transforming a variety of explants, such as freshly isolated or pre-cultured immature embryos, embryogenic callus and suspension cells. Also disclosed are methods for recovering transgenic plants after transformation within a short period of time, if the explants are regenerable at the time of transformation. Thus the frequency of somaclonal variation associated with prolonged in vitro culture period is significantly reduced. The transformation frequency using this system is comparable to or better than published methods using other systems, such as microprojectile bombardment.

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