Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Recombinant dna technique included in method of making a...
Reexamination Certificate
2006-10-17
2006-10-17
Kemmerer, Elizabeth (Department: 1647)
Chemistry: molecular biology and microbiology
Micro-organism, tissue cell culture or enzyme using process...
Recombinant dna technique included in method of making a...
C530S412000, C530S416000, C530S417000, C530S418000
Reexamination Certificate
active
07122344
ABSTRACT:
The present invention relates generally to the production of recombinant human uteroglobin (rhUG) for use as a therapeutic in the treatment of inflammation and fibrotic diseases. More particularly, the invention provides processes, including broadly the steps of bacterial expression and protein purification, for the scaled-up production of rhUG according to current Good Manufacturing Practices (cGMP). The invention further provides analytical assays for evaluating the relative strength of in vivo biological activity of rhUG produced via the scaled-up cGMP processes.
REFERENCES:
patent: 4691009 (1987-09-01), Palmer et al.
patent: 5266562 (1993-11-01), Mukherjee et al.
patent: 5696092 (1997-12-01), Patiemo et al.
patent: 2002/0006640 (2002-01-01), Ni et al.
patent: 2002/0160948 (2002-10-01), Pilon et al.
patent: 0617965 (1994-10-01), None
patent: WO 88/08983 (1988-11-01), None
patent: WO 96/09831 (1996-04-01), None
patent: WO 98/40657 (1996-12-01), None
Torkkeli et al. (1978), Uterine and lung uteroglobins in the rabbit. Two similar proteins with differential hormonal regulation. Biochim Biophys Act. 544(3): 578-592.
Scopes, R.K. (1994) Protein Purification Principles and Practice, 3rd Edition (Cantor, C.R., ed) pp. 270-277, Springer-Verlag, New York.
Mourot et al. (1989), Comparative Evaluation of Ultrafiltration Membranes for Purifications of Synthetic Peptides, Separation Science and Technology, 24(5 & 6): 353-367.
Mantile et al. (1993), Human Clara Cell 10-kDa Protein is the Counterpart of Rabbit Uteroglobin, J. Biol. Chem. 268(27): 20343-20351.
Miele et al. (1990), High Level Bacterial Expression of Uteroglobin, a Dimeric Eukaryotic Protein with Two Interchain Disulfide Bridges, in its Natural Quaternary Structure, J. Biol. Chem. 265(11): 6427-6435.
Peter et al. (1989), Recombinant rabbit uteroglobin expressed at high levels inE. coliforms stable dimers and binds progesterone, Protein Engineering 3(1): 61-66.
Andersson et al. (1994), Heterologous Expression of Human Uteroglobin/Polychlorinated Biphenyl-binding Protein, J. Biol. Chem. 269(29): 19081-19087.
Shin t al. (1997), Enhanced Production of Human Mini-Proinsulin in Fed-Batch Cultures at High Cell D nsity ofEscherichia coliBL21(DE3) [pET-3aT2M2], Biotechnol. Prog. 13: 249-257.
Torkkeli et al., “Uterine and lung uteroglobins it the rabbit. Two similar proteins with differential hormonal regulation”, Biochim Biophys Act. 1978, vol. 544, No. 3, pp. 578-592.
Andersson et al., “Heterologous Expression of Human Uteroglobin/Polychlorinated Biphenyl-binding Protein”, J. Biol. Chem., Jul. 22, 1994, vol. 269, No. 29, pp. 19081-19087.
Mourot et al., “Comparative Evaluation of Ultrafiltration Membranes for Purification of Synthetic Peptides”, Separation Science and Technology, 1989, vol. 24, No. 5 & 6, pp. 353-367.
Shin et al., “Enhanced Production of Human Mini-Proinsulin in Fed-Batch Cultures at High Cell Density ofEscherichia coliBL21 (DE3)[pET-3aT2M2]”, Biotechnol. Prog., 1997, vol. 113, pp. 249-257.
Pattabiraman et al., “Crystal Structure Analysis of Recombinant Human Uteroglobin and Molecular Modeling of Ligand Binding”, Ann. NY. Acad. Sci. 2000, vol. 923, pp. 113-127.
Pilon Aprile L.
Welch Richard W.
Borgeest Christina
Cittone, Esq. Henry
Claragen, Inc.
Evans, Esq. Barry
Kemmerer Elizabeth
LandOfFree
Methods for the production of purified recombinant human... does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Methods for the production of purified recombinant human..., we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Methods for the production of purified recombinant human... will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-3668190