Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Preparing oxygen-containing organic compound
Reexamination Certificate
2006-04-25
2006-04-25
Steadman, David J. (Department: 1656)
Chemistry: molecular biology and microbiology
Micro-organism, tissue cell culture or enzyme using process...
Preparing oxygen-containing organic compound
C435S137000
Reexamination Certificate
active
07033804
ABSTRACT:
The invention provides methods and host cells for the production of ascorbic acid intermediates. The invention also provides host cells having a modification in a polynucleotide that uncouples the catabolic pathway from the oxidative pathway by deleting the encoding for an endogenous enzymatic activity that phosphorylates D-glucose at its 6th carbon and/or a polynucleotide that has deleted the encoding for endogenous enzymatic activity that phosphorylates D-gluconate at its 6th carbon. Such host cells are used for the production of products, such as, ascorbic acid intermediates. Nucleic acid and amino acid sequences with inactivated enzymatic activity which phosphorylates D-glucose at its 6th carbon and inactivated enzymatic activity which phosphorylates D-gluconate at its 6th carbon are provided.
REFERENCES:
patent: 4757012 (1988-07-01), Estell et al.
patent: 4758514 (1988-07-01), Light et al.
patent: 4760025 (1988-07-01), Estell et al.
patent: 4767870 (1988-08-01), Fujiwara
patent: 5004690 (1991-04-01), Light et al.
patent: 5008193 (1991-04-01), Anderson et al.
patent: 5032514 (1991-07-01), Anderson et al.
patent: 5240843 (1993-08-01), Gibson et al.
patent: 5272073 (1993-12-01), Frost et al.
patent: 5376544 (1994-12-01), Lazarus et al.
patent: 5583025 (1996-12-01), Lazarus et al.
patent: 5686286 (1997-11-01), Fisher
patent: 5747306 (1998-05-01), Oka
patent: 5795761 (1998-08-01), Powers et al.
patent: 5939307 (1999-08-01), Wang et al.
patent: 5985617 (1999-11-01), Liao
patent: 6025184 (2000-02-01), Laffend et al.
patent: 0 955 368 (1999-11-01), None
patent: 1 087 015 (2001-03-01), None
patent: WO 92/18637 (1992-10-01), None
patent: WO 98/21340 (1998-05-01), None
patent: WO 98/59054 (1998-12-01), None
patent: WO 99/28480 (1999-06-01), None
patent: WO 99/55877 (1999-11-01), None
patent: WO 99/61623 (1999-12-01), None
patent: WO 01/12833 (2001-02-01), None
patent: WO 02/081631 (2002-04-01), None
patent: WO 02/081440 (2002-10-01), None
ATCC Bacteria and Bacteriophages, 19thEdition, 1996, p. 260.
Lavine et al. (1982) Mol Cell Biochem 48:45-58.
Branden et al. “Introduction to Protein Structure”, Garland Publishing Inc., New York, 1991, p. 247.
Dodge et al. (2001) Abstracts of Papers—American Chemical Society, 221st, BIOT-263.
Waleron et al. (2002) Microbiol 148 :583-595.
Seffernick et al. (1999) J Bacteriol 183:2405-2410.
Barredo et al. “Glucokinase-Deficient ofPenicillium chrysogenumIs Derepressed on Glucose Catabolite Regulation of Both β-Galactosidase and Penicillium Biosynthesis,” Antimicrob. Agents-Chemother 32: 1061-1067, (1988).
Bouvet, et al. “Taxonomic Diversity of the D-Glucose Oxidation Pathway in theEnterobacteriaceae,” International Journal of Systematic Bacteriology, 39:61-67, (1989).
Braun et al. “Immunogenic Duplex Nucleic Acids are Nuclease Resistant,” J. Immunol. 141: 2084-9, (1988).
Cha et al, “Identification and Characterization of aPantoea citreaGene Encoding Glucose Dehydrogenase That Is Essential for Causing Pink Disease of Pineapple,” Appl. Environ. Microbiol 63(1), 71-76 (1997).
Chaturvedi et al. “Stabilization of triple-stranded oligonucleotide complexes: use of probes containing alternating phosphodiester and stereo-uniform cationic phosphoramidate linkages,” Nucleic Acids Res. 24: 2318-23, (1996).
DiMarco et al “D-Glucose Transport System ofZymomonas mobilis,” Appl. Environ. Microbiol. 49:151-157, (1985).
Flores et al., “Pathway engineering for the production of aromatic compounds inEscherichia coli,” Nat. Biotechnol. 14: 620-623, (1996).
Frey et al., The Molecular biology of IncQ plasmids. In: Thomas (Ed.), Promiscuous Plasmids of Gram Negative Bacteria. Academic Press, London, pp. 79-94, (1989).
Frey et al., “Replication and copy number control of the broad-host-range plasmid RSF1010,” Gene 113:101-106, (1992).
Frohlich et al., “The primary structure of the years hexokinase Pll gene (HXK2) which is responsible for glucose repression,” Gene 36:105-111, (1985).
Fukuda et al., “Cloning of the Glucokinase Gene inEscherichia coli B” J. Bacteriol. 156:922-925, (1983).
Gerhardt, Philipp et al., ed., “Manual of Methods for General Bacteriology,” editor-in-chief R. G. E. Murray, editor, Morphology, Washington, D. C.: American Society for Microbiology, pp. 210-213, (1981).
Grindley et al., “Conversion of Glucose to 2-Keto-L-Gluconate, an Intermediate in L-Ascorbate Synthesis, by a Recombinant Strain ofErvinia citreus,” Applied and Environmental Microbiology 54: 1770-1775, (1988).
Harrod et al., “ Derepressed utilization of L-malic acid and succinic acid by mutants ofPachysolen tannophilus,” J. Ind. Microbiol. Biotechnol. 18:379-383, (1997).
Hoess et al., “Mechanism of Strand Cleavage and Exchange In the Cre-Iox Site-specific Recombination System,” J. Mol. Biol., 181:351-362, 1985.
Hoess et al., “Mechanism of Strand Cleavage and Exchange In the Cre-lox Site-specific Recombination System,” J. Mol. Biol., 181:351-362, 1985.
Kageyama et al. “Pantoea punctatasp. nov.,Pantoea citreasp. nov., andPantoea terrasp. nov. isolated from Fruit and Soil Samples,” International Journal of Systematic Bacteriology vol. 42, p. 203-210, (1992).
Kramer et al., “The gapped duplex DNA to oligonucleotide-directed mutation construction,” Nucleic Acids Res. 12:9441, (1984).
Kulbe et al. “Enzyme-Catalyzed Production of Mannitol and Gluconic Acid,” Ann. N.Y. Acad Sci 6:552, (Los Angeles), (1987).
Latimer et al. “Specificity of Monoclonal Antibodies Produced against Phosphorothioate and Ribo Modified DNAs,” Molec. Immunol. 32:1057-1064, (1995).
Lin E.C. C., “Glycerol Dissimilation and its regulation In bacteria,” Ann. Rev. Microbiol., 30:535-578, (1976).
Matsushita et al., “Membrane-Bound D-Gluconate Dehydrogenase from,” J. Biochem. 85:1173-1181, (1979).
Miller et al., “A Novel Suicide Vector and Its Use in Construction of Insertion Mutations: Osmoregulation of Outer Membrane Proteins and Virulence Determinants inVibrio choleraeRequires toxR,” J. Bacteriol. 170, 2575-2583, (1988).
Neijssel et al., “Physiological Significance and Bioenergetic Aspects of Glucose Dehydrogenase,” Antonie Van Leauvenhoek, 56(1):51-61, 1989.
Pachla et al, “Determination of Ascorbic Acid in Foodstuffs, Pharmaceuticals, and Body Fluids by Liquid Chromatography with Electrochemical Detection,” Anal. Chem. 48:364, (1976).
Palmeros et al., “A family of removable cassettes designed to obtain antibiotic-resistance-free genomic modifications ofEscherichia coliand other bacteria,” Gene 247, 255-264, (2000).
Parker et al., “Characterization of theZymomonas mobilisglucose facilitator gene product (glf) in recombinantEscherichia coli: examination of transport mechanism, kinetics and the role of glucokinase in glucose transport,” Mol. Microbiol. 15: 795-802, (1995).
Peyrottes et al., “Oligodeoxnucleoside phosphoramidates (P-NH2): synthesis and thermal stability of duplexes with DNA and RNA targets,” Nucleic Acids Res. 24: 1841-8, (1996).
Pujol et al, “gdhB, a gene encoding a second quinoprotein glucose dehydrogenase inPantoea citrea, is required for pink disease of pineapple,” Microbiol. 145, 1217-1226, (1999).
Pujol, et al, “Genetic and Biochemical Characterization of the Pathway inPantoea citreaLeading to Pink Disease of Pineapple,” J. of Bacterial. 182(8), 2000.
Reichstein and Grussner, “Eine ergiebige Synthese der I-Ascorbinasure (C-Vitamin)2),” Helv. Chem. Acta., 17, 311-328 (1934).
Russell et al “Carbohydrate Metabolism in the Mosquito PathogenBacillus sphaericus 232,” Appl. Environ. Microbiol., 55: 294-297, (1989).
Schultz et al. “Oligo-2′-fluoro-2′-deoxynucleotide N3′-P5′ phosphoramidates: synthesis and properties,” Nucleic
Dodge Timothy C.
Valle Fernando
Genencor International Inc.
Lynn Marcus-Wyner
Steadman David J.
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