Methods for the production of insulin in plants

Details Methods for the production of insulin in plants Methods for the production of insulin in plants

2004-06-17

2009-06-16

Worley, Cathy Kingdon (Department: 1638)

Multicellular living organisms and unmodified parts thereof and

Method of introducing a polynucleotide molecule into or...

Nonplant protein is expressed from the polynucleotide

C800S287000, C800S298000, C536S023510, C536S023400, C536S023500

Reexamination Certificate

active

07547821

ABSTRACT:
Commercial production of human insulin can be affected via transgenic expression in plant seeds. Thus, levels of insulin accumulation exceeding 0.1% of total cellular protein can be achieved recombinantly, through expression of the insulin with a single-chain antibody as a fusion partner. Production in seeds offers flexibility in storage and shipment of insulin as a raw material, and insulin retains its activity upon extraction from stored seed. Further, the amount of biomass subjected to extraction is limited, due to the relatively low water content of plant seeds.

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