Chemistry: molecular biology and microbiology – Vector – per se
Patent
1995-05-23
1998-10-20
Fox, David T.
Chemistry: molecular biology and microbiology
Vector, per se
435 691, 4351723, 435419, 536 236, 536 237, 536 241, 536 245, 47 58, 47DIG1, 800205, C12N 1582, C12N 1529, C12N 1531, A01H 102
Patent
active
058245421
ABSTRACT:
The present invention provides a dual method for producing male-sterile plants. Two genetically transformed plants, parents 1 and 2 are crossed to obtain male-sterile offspring. Parent 1 is transformed with an expression cassette comprising a nucleotide sequence encoding an anther-specific promoter which is operably linked to a nucleotide sequence encoding a transactivator. Parent 2 is transformed with an expression cassette comprising a target nucleotide sequence, which is capable of being activated by the transactivator, operably linked to a nucleotide sequence which encodes RNA or a polypeptide which will disrupt the formation of viable pollen. Therefore, crossing parent 1 with parent 2 results in male-sterile offspring. The male-sterile plants are useful for producing hybrid seed.
The invention also provides compositions and methods for high level expression of a coding region of interest in a plant.
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Crossland Lyle D.
Stein Jeffrey I.
Tuttle Annmarie
Fox David T.
Meigs J. Timothy
Novartis Finance Corporation
Pace Gary M.
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