Chemistry: molecular biology and microbiology – Differentiated tissue or organ other than blood – per se – or...
Patent
1996-09-30
1998-10-27
Saucier, Sandra E.
Chemistry: molecular biology and microbiology
Differentiated tissue or organ other than blood, per se, or...
435 12, 435 2, 435 13, A01N 102
Patent
active
058276408
ABSTRACT:
The present invention provides solutions and methods for preserving biological material that enable organs, tissues and cells to be stored for extended periods of time with minimal loss of biological activity. The inventive solutions are substantially isotonic with the biological material to be preserved and are substantially free of univalent oxyanions and of iodide. The solutions comprise a first neutral solute having a molecular weight of at least about 335 and a solubility in water of at least about 0.3M, and a second neutral solute having a molecular weight of less than about 200 and having both hydrophilic and hydrophobic moieties. The inventive solutions preferably contain CaSO.sub.4, together with combinations of anions and cations from the protein-stabilizing ends of the Hofmeister series, such as K.sub.2 SO.sub.4. In particular methods using solutions comprising raffinose or trehalose and trimethyl amine oxide or betaine, wherein the solution is substantially isotonic with the biological material and the solution is substantially free of iodide, dihydrogen phosphate, bicarbonate, nitrate and bisulfate are described.
REFERENCES:
patent: 4267269 (1981-05-01), Grode et al.
patent: 4380582 (1983-04-01), Orlando et al.
patent: 4476221 (1984-10-01), Kane et al.
patent: 4980277 (1990-12-01), Junnila
patent: 5084377 (1992-01-01), Rowan et al.
patent: 5200398 (1993-04-01), Strasberg et al.
patent: 5242792 (1993-09-01), Rudolph et al.
patent: 5571801 (1996-11-01), Segall et al.
Molinia et al., Effect of monosaccharides and disaccharides in Tris-based diluents on motility, acrosome integrity and fertility of pellet frozen ram spermatozoa., Animal Reproduction Sci. 36, 113-122, 1994.
Yano et al., Butyrate increases catalase activity and protects rat pulmonary artery smooth muscle cells against hyperoxia, Biochem. and Biophys. Research Commun 164(3), 1143-1148, 1989.
Staecker et al., Sodium butyrate preserves aspects of the differentiated phenotype of normal adult rate hepatocytes in culture, Journal of Cellular Physiology 135, 367-376, 1988.
Park et al., Effects of Cryoprotectants in minimizing physiochemical changes of bovine natural actomyosin during frozen storage, J. Food Biochem 11(2), 143-161, 1987.
Boutron et al., Reduction in Toxity for Red Blood Cells in Buffered Solutions containing high Concentrations of 2.3-Butanediol by Trehalose, Sucrose, Sorbitol or Mannitol, Cryobiology 31(4), 367-373, 1994.
Karow et al., Effects of Temperature, Potassium Concentration, and Sugar on Human Spermatozoa Motility: A Cell Preservation Model from Reproductive Medicine, Cryobiology 29, 250-254, 1992.
Newman et al., The Role of Trehalose and other Carbohydrates in Biopreservation, Biotechnology and genetic engineering reviews 11, 263-294, 1993.
Hogman et al., Red Cell Preservation in Protein-Poor Media, Vox Sanguinis 41, 274-281, 1981.
Stibenz, Preservation of Resuspended Red Cell Concentrates, Rate of Vesiculation and of Spontaneous Hemolysis, Folia haematologica. Internationales magazin fur klinische und morphologische hamatologie 114(4), 469-470, 1987.
Shier, Studies on the Mechanisms of Mammalian Cell Killing by a Freeze-Thaw Cycle: Conditions that Prevent Cell Killing Using Nucleated Freezing, Cryobiology 25, 110-120, 1988.
Brearley et al., A comparative study of the cryopreservation of human erythrocytes, ghosts and liposomes, Biochemical Society T ransaction 16, 354, 1988.
Goldstein et al., Enhanced transfection efficiency and improved cell survival after electroporation of G2/M-synchronized cells and treatment with sodium butyrate, Nucleic acid res. 17(10), 3959-71, 1989.
Fabre et al., Effects of different substances (sucrose, glucose, sorbitol and mannitol) on the resistance to deep freezing in liquid nitrogen of meristems from in vitro cultured carnations, Comptes rendus de l'Acadenie des Sciences Serie III, Sciences de la vie 304(20), 507-510. 1987.
Brass et al., Evaluation of University of Wisconsin Cold-Storage Solution in Warm Hypoxic Perfusion of Rat Liver: the Addition of Fractose Reduces Injury, Gastroenterology 105(5), 1455-1463, 1993.
Fremes et al., Cardiac Storage with UW Solution and Glucose, The Annals of thoracic surgery 58(5), 1368-1372, 1994.
Eschwege et al., Successful-4.degree. C. Liver Preservation in Rats with University of Wisconsin Solution and 2-3-Butanediol, Transplantation Proceedings 27(4), 2514-2515, 1995.
Minor et al., Effects of taurine on liver preservation in UW solution with consecutive ischemic rewarming in the isolated perfused rate liver, Transplantation international 8, 174-179, 1995.
Ahmad et al., Deep Freezing of Buffalo Bull Semen of Nili-ravi Breed, Indian Journal of Animal Health 22(2), 111-114, 1983.
Shier et al., Isotonic sucrose improves cryopreservation of cultured mammalian cells, In Vitro Cell Devel. Biol. 31, 336-337, 1995.
Strauss et al., SAG-sucrose medium for red blood cell preservation, Biomed. Biochem, Acta 46, S295-299, 1987.
Ferguson Alexander B.
Watson James D.
Wiggins Philippa M.
Biostore New Zealand Limited
Saucier Sandra E.
Sleath Janet
Speckman Ann W.
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