Methods for the preparation of endotoxin-binding proteins

Details Methods for the preparation of endotoxin-binding proteins Methods for the preparation of endotoxin-binding proteins

1993-05-19

1995-08-08

Draper, Garnette D.

Chemistry: molecular biology and microbiology

Micro-organism, tissue cell culture or enzyme using process...

Recombinant dna technique included in method of making a...

435 697, 4352402, 43524023, 435240243, 435244, 530412, 530416, C07K 122, C12N 1512

Patent

active

054398070

ABSTRACT:
Disclosed are improvements in methods for the isolation of endotoxin-binding proteins which are secreted by transfected host cells in appropriate cell culture media. In its preferred embodiment, the invention comprises addition of a cation exchange material to the media as the means of increasing the yield of recombinant endotoxin-binding proteins, such as bactericidal/permeability-increasing protein and lipopolysaccharide-binding protein.

REFERENCES:
patent: 5198541 (1993-03-01), Elsbach et al.
Gray et al. (1989), JBC vol. 264, pp. 9505-9509.
Schumann et al. (Sep. 1990), Science, vol. 249, pp. 1429-1431.
Ooi et al. (1987) JBC vol. 262, pp. 14891-14894.
Ion Exchange Chromatography, Principles and Methods p. 18, Pharmacia Fine Chemicals, 1983.

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