Methods for the preparation of bioactive peptides by protein hyd

Chemistry: natural resins or derivatives; peptides or proteins; – Proteins – i.e. – more than 100 amino acid residues – Chemical modification or the reaction product thereof – e.g.,...

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530300, 530343, 530344, A61K 3801, C07K 112

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active

058498826

ABSTRACT:
The present invention relates to improved methods for the production of bioactive peptides. Specifically the present invention relates to improved methods for the chemical hydrolysis of proteins by organic acids in the presence of metallic ions.

REFERENCES:
patent: 4720385 (1988-01-01), Lembach
Wei, C-H et al "Affinity Cleavage At The Putative Metal-Binding Site Of Pigeon Liver Malic Enzyme By The Fe.sup.2+ -Ascorbate System,"0 Biochemistry 33, 7931-7936 (1994).
Hutchens, T.W. et al "Recognition Of Transition Metal Ions By Peptides: Identification Of Specific Metal-Binding Peptides In Proteolytic Digest Maps By UV Laser Desorption Time-Of-Flight Mass Spectrometry," FEBS Letters 10582 296:1, 99-102 (1992).
Chiou, S-H., "DNA-And Protein-Scission Activities Of Oscorbate In The Presence Of Copper Ion And A Copper-Peptide Complex," J. Biochem. 94, 1259-1267 (1983).
Misono, K.S., "Acidic pH-And Metal Ion (Zn.sup.++ Or Mn.sup.++ -Dependent Proteolysis Of 140 kDa Atrial Natriuretic Factor Receptor In Bovine Adrenal Cortex Plasma Membranes: Evidence For Membrane-Bound Acidic Metalloendopeptidase," Biochemical and Biophysical Research Communications 152:2, 658-667 (1988).

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