Methods for the manufacture of pure single enantiomer...

Details Methods for the manufacture of pure single enantiomer... Methods for the manufacture of pure single enantiomer...

2011-07-19

2011-07-19

Liu, Sue (Department: 1639)

Combinatorial chemistry technology: method, library, apparatus

Method of screening a library

By measuring catalytic activity

C435S069100, C435S183000

Reexamination Certificate

active

07981844

ABSTRACT:
The invention provides biocatalytic methods for the manufacture of pure single enantiomer compounds. This invention provides methods of screening for enzymes which are highly enantioselective or enzymes that can provide any desired stereoisomer of a compound. The invention provides the use of single enantiomer substrates in performing a growth screen of a clonal library to identify highly stereoselective enzymes. In one aspect, methods for screening and identification of enzymes, e.g., transaminases, nitrilases, aldolases, epoxide hydrolases are provided. Methods for the production and screening of gene libraries generated from nucleic acids isolated from more than one organism for enzyme, e.g., transaminase, activities are also provided.

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