Methods for the isothermal amplification of nucleic acid molecul

Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Preparing compound containing saccharide radical

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435 6, C12Q 168, C12P 1934

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active

053546687

ABSTRACT:
Methods for amplifying a nucleic acid molecule which employs a single primer, and in which the amplification is performed under isothermal conditions. The invention also includes kits containing reagents for conducting the method.

REFERENCES:
patent: 4582788 (1986-04-01), Erlich
patent: 4673640 (1987-06-01), Backman
patent: 4683194 (1987-07-01), Saiki et al.
patent: 4683202 (1987-07-01), Mullis
patent: 4959317 (1990-09-01), Sauer
Bellofatto, et al. Generation of a Tn5 promoter probe and its use in the study of gene expression . . . Proc. Natl. Acad Sci. USA (Feb. 1984) 81:1035-39.
Mullis et al., "Specific Enzymatic Amplification of DNA in Vitro: The Polymerase Chain Reaction," Cold Spring Harbor Symp. Quant. Biol. 51:263-273 (1986).
Higuchi, "Using PCR to Engineer DNA," In: PCR Technology, Ehrlich, H. (ed.), Stockton Press, N.Y., 1989, pp. 61-68.
Hoess, R. et al., "The Nature of the Interaction of the P1 Recombinase Cre with the Recombining Site loxP," Cold Spring Harbor. Symp. Quant. Biol. 49:761-768 (1984).
Hamilton, D. L. et al., "Site-Specific Recombination by the Bacteriophage P1 loxP-Cre System," J. Molec. Biol. 178:481-486 (1984).
Sauer, B. "Functional Expression of the cre-lox Site-Specific Recombination System in the Yeast Saccharomyces cerevisiae," Molec. Cell. Biol. 7:2087-2096 (1987).
Sauer, B. et al. "Site-Specific DNA Recombination in Mammalian Cells by the Cre Recombinase of Bacteriophage P1," Proc. Natl. Acad. Sci. (U.S.A.) 85:5166-5170 (1988).
Abremski, K. et al., "Studies on the Properties of P1 Site-Specific Recombination: Evidence for Topologically Unlinked Products Following Recombination," Cell 32:1301-1311 (1983).
Hoess, R. et al., "P1 Site-Specific Recombination: Nucleotide Sequence of the Recombining Sites," Proc. Natl. Acad. Sci. (U.S.A.) 79:3398-3402 (1982).
Sternberg, N. et al., "Site-Specific Recombination and Its Role in the Life Cycle of Bacteriophage P1," Cold Spring Harbor Symp. Quant. Biol. 45:297-309 (1981).
Hoess, R. et al., "Interaction of the Bacteriophage P1 Recombinase Cre with the Recombining Site loxP," Proc. Natl. Acad. Sci. (U.S.A.) 81:1026-1029 (1984).
Abremski, K. et al., "Bacteriophage P1 Site-Specific Recombination: Purification and Properties of the Cre Recombinase Protein," J. Molec. Biol. 259:1509-1514 (1984).
Abremski, K. et al., "Bacteriophage P1 Cre-loxP Site-Specific Recombination: Site-Specific DNA Topoisomerase Activity of the Cre Recombination Protein," J. Biol. Chem. 261:391-396 (1986).
Sauer, B. et al., "Site-Specific Insertion of DNA into a Pseudorabies Virus Vector," Proc. Natl. Acad. Sci. (U.S.A.) 84:9108-9112 (1987).
Palazzolo, M. J. et al., "Phage Lambda cDNA Cloning Vectors for Subtractive Hybridization, Fusion-Protein Synthesis and Cre-loxP Automatic Plasmid Subcloning," Gene 88:25-36 (1990).
Kwoh D. et al., "Transcription-Based Amplification System and Detection of Amplified Human Immunodeficiency Virus Type 1 with a Bead-Based Sandwich Hybridization Assay," Proc. Natl. Acad. Sci. (U.S.A.) 86:1173 (1989).
Ohara, O. et al., "One-sided Polymerase Chain Reaction: The Amplification of cDNA," Proc. Natl. Acad. Sci. (U.S.A.) 86:5673-5677 (1989).
Frohman, M. A., "RACE: Rapid Analysis of cDNA Ends," In: PCR Protocols: A Guide to Methods and Applications Academic Press, N.Y. (1990).
Wu, D. Y. et al., "The Ligation Amplification Reaction (LAR)-Amplification of Specific DNA Sequences Using Sequential Rounds of Template-Dependent Ligation," Genomics 4:560 (1989).
Mullis, K. B. et al., "Specific Synthesis of DNA in Vitro via a Polymerase-Catalyzed Chain Reaction," In: Met. Enzymol. 155:335-350 (1987).
Walker, G. T. et al., "Isothermal in vitro Amplification of DNA by a Restriction Enzyme/DNA Polymerase System," Proc. Natl. Acad. Sci. (U.S.A.) 89:392-396 (1992).
Chatterjee, D. K. et al., "Cloning and Overexpression of the Gene Encoding Bacteriophage T5 DNA Polymerase," Gene 97:13-19 (1991).
Gillin, F. et al., "Control of Mutation frequency by Bacteriophage T4 DNA Polymerase: I. The CB120 Antimutator DNA Polymerase is Defective in Strand Displacement," J. Biol. Chem 251:5219-5224 (1976).
Kolodner, R. et al., "Gene 4 Protein of Bacteriophage T7: Characterization of the Product Synthesized by the T7 DNA Polymerase and Gene 4 Protein in the Absence of Ribonucleoside 5'-Triphosphates," J. Biol. Chem 253:574-584 (1978).
Eki, T. et al., "Influence of Poly(ADP-ribose) Polymerase on the Enzymatic Synthesis of SV40 DNA," J. Biol. Chem 266:3087-3100 (1991).
Parada, C. et al., "Transcriptional activation of pBR322 DNA can lead to Duplex DNA Unwinding Catalyzed by the Escherichia coli Preprimosome," J. Biol. Chem 264:15120-15129 (1989).

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