Chemistry: molecular biology and microbiology – Process of mutation – cell fusion – or genetic modification – Introduction of a polynucleotide molecule into or...
Patent
1999-05-12
2000-11-28
LeGuyader, John L.
Chemistry: molecular biology and microbiology
Process of mutation, cell fusion, or genetic modification
Introduction of a polynucleotide molecule into or...
424450, 4353201, 435458, C12N 1563, C12N 1588, A61K 9127
Patent
active
06153434&
ABSTRACT:
The subject invention concerns novel materials and methods for the delivery of substances, such as DNA or polypeptides, into cells. In a specific embodiment, substances are delivered into cells using a novel class of lipid compounds. These compounds, cationic lipid compounds having a disulfide bond, can be complexed with DNA to be inserted into a cell in gene therapy. Once inside the cell, enzymes present within the cell cleave the disulfide bond and the DNA is released.
REFERENCES:
patent: 5656609 (1997-08-01), Wu et al.
patent: 5942634 (1999-08-01), Siegel et al.
Boutorine et al. (Biochimie, vol. 75, 35-41), 1993.
Moradpour, D. et al., (1996) "Efficient Gene Transfer into Mammalian Cells With Cholesteryl-Spermidine" Biochemical and Biophysical Research Communications, 221: 82-88.
Shen, W.C. et al., (1989) "The Intracellular Release of Methotrexate From A Synthetic Drug Carrier System Targeted to Fc Receptor-Bearing Cells" Journal of Controlled Release, 10: 89-96.
Briscoe, Page et al., (1995) "Delivery Of Superoxide Dismutase To Pulmonary Epithelium Via pH-sensitive Liposomes" American Journal of Physiology, 268 (Lung Cellular and Molecular Physiology, 12): L374-L380.
Wang, J., Szoka, F.C.Jr., (1995) "Synthesis Of Mutlivalent Cationic Cholesteryl Lipids For Use As Gene Delivery Vehicles" Proceed Intern. Symp. Control.Rel. Bioact. Mater., 22: 414-415.
Tang, F., Hughes, J.A. (1998) "Introduction Of A Disulfide Bond Into A Cationic Lipid Enhances Transgene Expression Of Plasmid DNA" Biochemical and Biophysical Research Communications 242: 141-145.
Shen, W.C., (1986) "Disulfide And Other Spacers For The Intracellular Release Of Drugs From Polymeric Carriers" Polymer Preprints, 27(1): 9-10.
Kostina, E.V., A.S. Boutorine (1993) "Reversible covalent attachment of cholesterol to oligodeoxy-ribonucleotides for studies of the mechanisms of their penetration into eucaryotic cells" Biochimie 75:35-41.
Caplen, N.J. et al. (1995) "In vitro liposome-mediated DNA transfection of epithelial cell lines using the cationic liposome DC-Col/DOPE" Gene Therapy 2:603-613.
Farhood, Hassan, Remo Bottega, Richard M. Epand, Leaf Huang (1992) "Effect of cationic cholesterol derivatives on gene transfer and protein kinase C activity" Biochemica et Biophysica Acta, 1111:239-246.
Felgner et al., (1994) "Enhanced Gene Delivery and Mechanism Studies with a Novel Series of Cationic Lipid Formulations" Journal of Biological Chemistry 269(4):2550-2561.
Gao, Xiang, and Leaf Huang (1991) "A Novel Cationic Liposome Reagent For Efficient Transfection Of Mammalian Cells" Biochemical and Biophysical Research Communication 179(1):280=285.
Hermanson, G.T. (1996) In: Bioconjugate Techniques, pp. 150-152, Academic Press, San Diego.
Legendre, J.Y., A. Trzeciak, B. Bohrmann, U. Deuschle, E. Kitas, A. Supersaxo (1997) "Dioleoylmelittin as a Novel Serum-Insensitive Reagent for Efficient Transfection of Mammalian Cells" Bioconjugate Chem. 8:57-63.
Liu, Feng, Jingping Yang, Leaf Huang, Dexi Liu (1996) "New Cationic Lipid Formulations for Gene Transfer" Pharmaceutical Research 13(12):1856-1860.
Trail, P.A., D. Willner, S.J. Lasch, A.J. Henderson, S. Hofstead, A.M. Casazza, R.A. Firestone, I. Hellstrom, K.E. Hellstrom (1993) "Cure of Xenografted Human Carcinomas by BR96-Doxorubicin Immunoconjugates" Science 261:212-215.
Trail et al. (1997) "Effect of Linker Cariation of the Stability, Potency, and Efficacy of Carcinoma-reactive BR64-Doxorubicin Immunoconjugates" Cancer Research 57:100-105.
Vigneron et al. (1996) "Guanidinium-cholesterol cationic lipids: Efficient vectors for the transfection of eukaryotic cells" Proc. Natl. Acad. Sci. USA 93:9682-9686.
Zabner, Joseph, Al J. Fasbender, Tom Moninger, Kristi A. Poellinger, Michael J. Welsh (1995) "Cellular and Molecular Barriers to Gene Transfer by a Cationic Lipid" The Journal of Biological Chemistry 270(32):18997-19007.
Hassner, Alfred, Vazken Alexanian (1978) "Direct Room Temperature Esterification Of Carboxylic Acids" Tetrahedron Letters 46:4475-4478.
Itoh, Masumi et al. (1977) "Peptides: VI. Some Oxime Carbonates as Novel t-Butoxycarbonylating Reagents" Bulletin of the Chemical Soc. of Japan 50(3):718-721.
Aberle, Alfred M., Fern Tablin, Ji Zhu et al. (1998) "A Novel Tetraester Construct That Reduces Cationic Lipid-Associated Cytotoxicity Implications for the Onset of Cytotoxicity" Biochemistry 37:6533-6540.
Felgner, Philip L., Thomas R. Gadek, Marilyn Holm et al. (1987) "Lipofection: A highly efficient, lipid-mediated DNA-transfection procedure" Proc. Natl. Acad. Sci. USA 84:7413-7417.
Lee, Robert J., Leaf Huang (1997) "Lipidic Vector Systems for Gene Transfer" Therapeutic Drug Carrier Systems 14(2):173-206.
Escriou, V., C. Ciolina, A. Helbling-Leclerc, P. Wils, D. Scherman (1998) "Cationic lipid-mediated gene transfer: Analysis of cellular uptake and nuclear import of plasmid DNA" Cell Biology and Toxicology 14:95-104.
Tang, Fuxing and Jeffrey A. Hughes (1998) "Introduction of a Disulfide Bond into a Cationic Lipid Enhances Transgene Expression of Plasmid DNA" Biochemical and Biophysical Research Communications 242:141-145.
Gao, X. and L. Huang (1995) "Cationic liposome-mediated gene transfer" Gene Therapy 2:710-722.
Behr, Jean Paul (1989) "Photohydrolysis of DNA by Polyaminobenzenediazonlum Salts" J. Chem. Soc. Chem. Commun. 101-103.
Rolland, Alain P. (1998) "From Genes to Gene Medicines: Recent Advances in Nonviral Gene Delivery" Critical Reviews in Therapeutic Drug Carrier Systems 15(2):143-198.
Hughes Jeffrey Allen
Tang Fuxng
LeGuyader John L.
Nguyen Dave Trong
University of Florida
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