Methods for the inactivation of viruses in viral-contaminated ph

Chemistry: molecular biology and microbiology – Virus or bacteriophage – except for viral vector or... – Inactivation or attenuation; producing viral subunits

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435236, 514 21, 424530, 424 943, 530381, 530384, 530380, C12N 706, C12N 704, A61K 3514, C07K 1504

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053004337

ABSTRACT:
The present invention provides a fail-safe combination of chemical and physical means for rendering a blood product which comprises a labile blood protein free of viruses without incurring protein denaturation. The blood product is contacted with an effective amount of a selected chemical disinfectant, preferably, sodium thiocyanate in combination with a physical process, preferably, ultrafiltration. The blood product may be plasma, serum, plasma concentrate, cryoprecipitate, cryosupernatant, plasma fractionation precipitate or plasma fractionation supernatant containing viruses such as hepatitis or human immunodeficiency virus.

REFERENCES:
patent: 4540573 (1985-09-01), Neurath et al.
patent: 4673733 (1987-06-01), Chandra et al.
patent: 4732683 (1988-03-01), Georgiades et al.
Almeida et al, J. Med. Vir., 4:269-277 (1979).
Limentani et al, Blood, vol. 70, No. 5 (Nov.), 1987 pp. 1312-1315.
Austen et al, Thromb Haemostas 48(1) 46-48 (1982).

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