Methods for the in vitro detection and identification of unknown

Details Methods for the in vitro detection and identification of unknown Methods for the in vitro detection and identification of unknown

1984-06-11

1987-03-24

Warden, Robert J.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving virus or bacteriophage

435 6, 435259, 435803, 935 77, 935 78, C12Q 170, C12Q 168, C12N 106

Patent

active

046525173

ABSTRACT:
Methods for the detection and identification of unknown pathogens or genetic entities from samples suspected of containing the same involve the use of novel wicking techniques whereby sample DNA/RNA becomes readily available on a porous, inert, positively charged support in single stranded form for hybridization with a hybridization probe having a nucleotide sequence substantially complementary to a nucleotide sequence of the sample DNA/RNA. Such methods permit the detection and identification of unknown pathogens and genetic entities in a shorter period of time than that required by conventional methods. Lysis solutions for rapidly lysing bacterial cells without the aid of enzymes are also disclosed.

REFERENCES:
patent: 4358535 (1982-11-01), Falkow
patent: 4407942 (1983-10-01), Birnboim
Moseley, S. L. et al, J. Infect. Dis., vol. 145, No. 6, 1982, pp. 863-869.
Windholz, M. et al, The Merck Index, 10th edition, Merck & Co., Inc., N.J., 1983, p. 1204.

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