Methods for the identification of compounds capable of inducing

Details Methods for the identification of compounds capable of inducing Methods for the identification of compounds capable of inducing

1994-09-21

1998-06-09

Adams, Donald E.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving antigen-antibody binding, specific binding protein...

435 78, 435 691, 4351723, G01N 3353, C12P 2106, C12N 1500

Patent

active

057631905

ABSTRACT:
Human immunodeficiency virus (HIV)-1, HIV-2, and simian immunodeficiency virus contain, in addition to the canonical gag/pol/env genes, additional small open reading frames encoding gene products, including the 96-amino acid 15-kDa virion associated HIV-1 Vpr gene product. The conservation of the vpr open reading frame in primate lentiviruses suggests that vpr is critical to viral replication. A biologically active recombinant HIV-1 Vpr protein was employed as a ligand to identify its cellular targets. A novel 41-kDa cytosolic protein was identified and termed the viral protein R interacting protein, or Rip-1. Rip-1 displays a wide tissue distribution, including relevant targets of HIV infection. Vpr protein induced nuclear translocation of Rip-1, as did glucocorticoid receptor (GR)-II-stimulating steroids. Vpr and Rip-1 coimmunoprecipitated with the human GR as part of a receptor complex. The present invention discloses methods for the identification of compounds capable of inducing GR-II/Rip-1 receptor complex cytosolic to nuclear translocation.

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