Methods for the identification and characterization of reticuloc

Details Methods for the identification and characterization of reticuloc Methods for the identification and characterization of reticuloc

1991-12-05

1994-11-01

Housel, James C.

Chemistry: analytical and immunological testing

Biological cellular material tested

436 10, 436172, 436800, 435 29, 435 39, G01N 3348

Patent

active

053607392

ABSTRACT:
Methods for characterizing and distinguishing reticulocytes and mature red blood cells use reagent compositions which include an organic cationic dye for staining the reticulocytes in the blood sample and a buffer solution for maintaining a pH of about 6 to about 9. The dyes may be the red excitable fluorescent dye Oxazine 750, or the blue excitable fluorescent dyes Acridine Orange or derivatives of Acridine Orange. When a zwitterionic surfactant is included in the reagent composition for isovolumetric sphering of the reticulocytes and erythrocytes, and the reagent composition and whole blood sample mixture is passed through the sensing region of a flow cytometer, the light scattered through at least one angular interval and that fluoresced by each cell is measured, the erythrocytes can be distinguished from reticulocytes and the volume, hemoglobin concentration and the hemoglobin content of each reticulocyte or erythrocyte, and the mean cell volume, mean corpuscular hemoglobin concentration, and mean cell hemoglobin of the reticulocytes and/or erythrocytes are calculated from the measured cell-by-cell volume and hemoglobin concentration.

REFERENCES:
patent: 3684377 (1972-08-01), Adams et al.
patent: 3883247 (1975-05-01), Adams
patent: 4325706 (1982-04-01), Gershman et al.
patent: 4336029 (1982-06-01), Natale
patent: 4412004 (1983-10-01), Ornstein et al.
patent: 4490353 (1984-12-01), Crawford et al.
patent: 4571388 (1986-02-01), O'Connel et al.
patent: 4575490 (1986-03-01), Ornstein et al.
patent: 4707451 (1987-11-01), Sage, Jr.
patent: 4735504 (1988-04-01), Tycko
patent: 4883867 (1989-11-01), Lee et al.
patent: 4971917 (1990-11-01), Kuroda
patent: 4981803 (1991-01-01), Kuroda
patent: 4985174 (1991-01-01), Kuroda et al.
patent: 5039613 (1991-08-01), Matsuda et al.
patent: 5075556 (1991-12-01), Fan et al.
Muirhead et al. "Flow Cytometry: Present and Future" Apr. 1985, pp. 339 and
Harlow Ed and Lane David, "Antibodies a Laboratory Manual", 1988 p. 683.
Aldrich, Catalog of Fine Chemicals 1988-1989 p. 1097.
Cytometry, vol. 7 (1986) "Flow Cytometry of DNA Content Using Oxazine 750 or Related Laser Dyes with 633 nm Excitation", Shapiro, et al., pp. 107-110.

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