Chemistry: analytical and immunological testing – Biological cellular material tested
Patent
1991-12-05
1994-09-27
Housel, James C.
Chemistry: analytical and immunological testing
Biological cellular material tested
436 10, 436 17, 436172, 436800, G01N 3348
Patent
active
053506952
ABSTRACT:
Methods for characterizing and distinguishing reticulocytes and mature red blood cells use reagent compositions which include organic cationic dyes for staining the reticulocytes in the blood sample and buffer solutions for maintaining a pH of about 6 to about 9. The dyes may be the blue absorption dyes Oxazine 750 or New Methylene Blue. When a zwitterionic surfactant is included in the reagent composition for isovolumetric sphering of the reticulocytes and erythrocytes, and the reagent composition and whole blood sample mixture is passed through the sensing region of a flow cytometer, the light scattered and absorbed by each cell is measured, the erythrocytes can be distinguished from reticulocytes and the volume, hemoglobin concentration and the hemoglobin content of each reticulocyte or erythrocyte, and the mean cell volume, mean corpuscular hemoglobin concentration, and mean cell hemoglobin of the reticulocytes or erythrocytes are calculated from the measured cell-by-cell volume and hemoglobin concentration. One method also includes adjusting the measured absorption signals for pseudo-absorption.
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Ben-David Daniel
Colella Gregory M.
Cupo Albert
Fan Sophie S.
Fischer Gena
Housel James C.
Miles Inc.
Mount Sinai School of Medicine of the City University of New Yor
Tran Lien
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