Methods for the diagnosis and treatment of insulin-dependent dia

Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving antigen-antibody binding – specific binding protein...

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435 792, 436501, 436506, 530351, 530350, G01N 3353, G01N 33564, C07K 100

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active

060226978

ABSTRACT:
Methods for detecting the status of an insulin-dependent diabetes mellitus (IDDM)-associated autoimmune response in a mammal are provided. Specifically, the ratio of the frequency of T helper 1 cells to T helper 2 cells specific for a pancreatic .beta.-cell associated antigen is indicative of the status of the autoimmune response. The methods may be employed prior to the onset of the clinical symptoms of the disease, thereby allowing identification of those at risk for developing clinical symptoms of IDDM, or subsequent to pancreatic tissue transplantation, for example, to measure the efficacy of treatment directed to enhancing the lifetime of the tissue transplant. Methods for prolonging the survival of tissue transplants are also provided. Specifically, a tissue-associated antigen is administered to the mammal which serves to shift the pathogenic Th1 response associated with pathological immunity toward a protective Th2 response.

REFERENCES:
Liblau, RS et al. Immunology Today. 16:34-38, 1995.
Tian, J et al. J. Exp. Med. 183:1561-1567, Apr. 1996.
Rocken et al., Immunology Today 17(5):225-231 (1996).
Mosmann et al., Immunology Today 17(30:138-146 (1996).
Tisch, Roland et al., "Immune Response to Glutamic Acid Decarboxylase Correlates with Insulitis in non-obese diabetic mice", Nature, vol. 366, pp. 72-75 (Nov. 4, 1993).
Kaufman, Daniel L. et al., "Spontaneous Loss of T-cell Tolerance to Glutamic Acid Decarboxylase in Murine Insulin-Dependent Diabetes", Nature, vol. 366, pp. 69-72 (Nov. 4, 1993).

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